Human IL-5 ELISA Kit (ab215536)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.3 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-5 ELISA Kit
IL-5 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5 8.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 5.4% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.3 pg/ml -
検出範囲
4.7 pg/ml - 300 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 105 104% - 107% Urine 81 80% - 81% Serum 96 93% - 100% Hep Plasma 99 97% - 100% EDTA Plasma 94 93% - 96% Cit plasma 93 90% - 96% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Cow -
製品の概要
Human IL-5 ELISA Kit (ab215536) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-5 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-5 with 3.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human IL-5 Capture Antibody 1 x 600µl 10X Human IL-5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
配列類似性
Belongs to the IL-5 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- B-cell differentiation factor I
- Colony stimulating factor
- EDF
see all -
参照データベース
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
関連製品
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Alternative Versions
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ELISA kits
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard curve comparison between human IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 149 pg/mL in serum, 131 pg/mL in plasma (citrate), 165 pg/mL in plasma (heparin), 142 pg/mL in plasma (EDTA) and 133 pg/mL in urine.
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Conditioned media was harvested after 48 hours. IL-5 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-5 were measured in duplicate, interpolated from the IL-5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 226.9 pg/mL in PHA stimulated PBMC supernatant and 1.94 pg/mL in unstimulated PBMC supernatant.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab215536 は 1 報の論文で使用されています。
- Yamamoto M et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). PubMed: 29385874