Anti-Cathepsin B 抗体 [EPR21033] (ab214428)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21033] to Cathepsin B
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Cathepsin B antibody [EPR21033]
Cathepsin B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21033] to Cathepsin B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse kidney tissue lysate; Rat thymus and kidney tissue lysates; RAW 264.7 and PC-12 whole cell lysates. IHC-P: Mouse and rat kidney tissues. ICC/IF: RAW 264.7 and PC-12 cells. Flow Cyt (intra): RAW 264.7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21033 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214428の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/500.
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IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (2) |
1/1000. Predicted molecular weight: 37 kDa.
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ICC/IF | (1) |
1/250.
|
特記事項 |
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Flow Cyt (Intra)
1/500. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/250. |
ターゲット情報
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機能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 13030 Mouse
- Entrez Gene: 64529 Rat
- SwissProt: P10605 Mouse
- SwissProt: P00787 Rat
- Unigene: 236553 Mouse
- Unigene: 472420 Mouse
- Unigene: 100909 Rat
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別名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
画像
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All lanes : Anti-Cathepsin B antibody [EPR21033] (ab214428) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 25,31,43 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,2: 23 seconds; Lane 3: 50 seconds; Lane 4: 15 seconds; Lane 5: 2 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile and molecular mass is consistent with what has been described in the literature (PMID:20536394; PMID: 10447678).
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin B with ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on mouse kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cathepsin B with ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on rat kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling Cathepsin B with ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunofluorescent analysis of 100% methanol-fixed PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Cathepsin B with ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on PC-12 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling Cathepsin B with ab214428 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (11)
ab214428 は 11 報の論文で使用されています。
- Li Z et al. Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting Proteostasis. Int J Biol Sci 18:2018-2031 (2022). PubMed: 35342336
- Wang Y et al. α-Lipoic Acid-Plus Ameliorates Endothelial Injury by Inhibiting the Apoptosis Pathway Mediated by Intralysosomal Cathepsins in an In Vivo and In Vitro Endothelial Injury Model. Oxid Med Cell Longev 2022:8979904 (2022). PubMed: 35450412
- Liudkovska V et al. TENT5 cytoplasmic noncanonical poly(A) polymerases regulate the innate immune response in animals. Sci Adv 8:eadd9468 (2022). PubMed: 36383655
- Soares AC et al. PIKfyve activity is required for lysosomal trafficking of tau aggregates and tau seeding. J Biol Chem 296:100636 (2021). PubMed: 33831417
- Zhang L et al. MicroRNA-140-5p represses chondrocyte pyroptosis and relieves cartilage injury in osteoarthritis by inhibiting cathepsin B/Nod-like receptor protein 3. Bioengineered 12:9949-9964 (2021). PubMed: 34565303