Human IL-8 ELISA Kit (ab214030)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.8 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human IL-8 ELISA Kit
IL-8 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5 1.8% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 7.5% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.8 pg/ml -
検出範囲
3.91 pg/ml - 250 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 99 93% - 103% Cell culture media 101 98% - 104% Hep Plasma 87 82% - 91% EDTA Plasma 88 84% - 92% Cit plasma 86 83% - 90% Cerebral Spinal Fluid 89 84% - 94% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Cow -
製品の概要
Human IL-8 ELISA Kit (ab214030) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-8 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-8 with 1.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only.
Cell and tissue extract samples have not been tested with this kit.
SPECIES REACTIVITY
This kit recognizes human IL-8 protein.
Other species reactivity was determined by measuring 100% mouse, rat, and bovine serum samples, interpolating the IL-8 protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the IL-8 protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
CALIBRATION
This immunoassay is calibrated against a highly purified human IL-8. The NIBSC/WHO unclassified purified human IL-8 preparation 89/520 was evaluated in this kit.
The dose response curve of the unclassified standard IL-8 parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human IL-8 kit to approximate NIBSC IU/mL units, use the equation below.
NIBSC (89/250) approximate value (IU/mL) = 0.0042 x SimpleStep Human IL-8 value (pg/mL).
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特記事項
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Human IL-8 Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Human IL-8 Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 4BI 1 x 6ml 10 x 6ml 1 x 6ml Human IL-8 Lyophilized Recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seals 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究分野
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機能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
細胞内局在
Secreted. - Information by UniProt
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別名
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
参照データベース
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
関連製品
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Alternative Versions
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ELISA kits
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KO cell lines
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.25% stimulated PBMC supernatant. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Example of human IL-8 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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Standard curve comparison between human IL-8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA® kit shows a 3-fold increase in sensitivity.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 61.6 pg/mL with a range of 62 – 174.9 pg/mL; two individuals (Donor #3 and Donor #9) measured below the minimal detectable dose.
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Background-subtracted data values (mean +/- SD) are graphed.
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The IL-8 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 63.2 pg/mL in serum.
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The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 1:400 and stimulated 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Measured values were interpolated from the IL-8 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. The mean IL-8 concentration was determined to be 2.2 ng/mL in unstimulated, 119.7ng/mL in stimulated, and undetectable in media.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native IL-8 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Recombinant IL-8 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Human IL-8 concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-8 ELISA kit (ab214030). Wild-type PC-3 cells and CXCL8 knockout PC-3 cells (ab273743) were assessed in duplicate (n=2) and were either treated with 2 µg/ml LPS for 6 hours to induce expression of IL-8 or not treated with LPS. Data are represented as the mean and error bars represent standard deviation.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab214030 は 22 報の論文で使用されています。
- Guo X et al. Naringin attenuates acute myocardial ischemia-reperfusion injury via miR- 126/GSK-3β/β-catenin signaling pathway. Acta Cir Bras 37:e370102 (2022). PubMed: 35416858
- Tsai T et al. Changes of Subjective Symptoms and Tear Film Biomarkers following Femto-LASIK. Int J Mol Sci 23:N/A (2022). PubMed: 35886858
- Tadala L et al. Infection-induced membrane ruffling initiates danger and immune signaling via the mechanosensor PIEZO1. Cell Rep 40:111173 (2022). PubMed: 35947957
- Bai X et al. The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis. BMC Immunol 23:42 (2022). PubMed: 36088289
- Zhang X et al. Extracellular Vesicles Derived from Lung Cancer Cells Induce Transformation of Normal Fibroblasts into Lung Cancer-Associated Fibroblasts and Promote Metastasis of Lung Cancer by Delivering lncRNA HOTAIR. Stem Cells Int 2022:3805013 (2022). PubMed: 36267242