Human Galectin-4 ELISA Kit (ab213785)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 31.2 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Galectin-4 ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% 1 16 209pg/ml 11.7 = 7.6% 2 16 1291pg/ml 98.11 = 7.6% 3 16 4066pg/ml 223.63 = 5.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% 1 24 215pg/ml 12.04 = 5.6% 2 24 1324pg/ml 108.56 = 8.2% 3 24 4394pg/ml 320.76 = 7.3% -
サンプルの種類
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
31.2 pg/ml - 2000 pg/ml -
全工程の試験時間
3h 30m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The Human Galectin-4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213785) is designed for the quantitative measurement of Human Galectin-4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: A2-I323) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Galectin-4 amount of sample captured in plate.
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特記事項
Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly under expressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Galectin-4 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human Galectin-4 antibody 1 x 100µl Lyophilized recombinant Human Galectin-4 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究分野
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機能
Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. -
配列類似性
Contains 2 galectin domains. -
ドメイン
Contains two homologous but distinct carbohydrate-binding domains. - Information by UniProt
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別名
- Antigen NY CO 27
- Antigen NY-CO-27
- Antigen NYCO27
see all -
参照データベース
- Entrez Gene: 3960 Human
- Omim: 602518 Human
- SwissProt: P56470 Human
- Unigene: 5302 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab213785 は 1 報の論文で使用されています。
- Abdel-Tawab MS et al. Evaluation of Serum and Gene Expression of Galectin-4, Interleukin-27, and Complement-7 in Hepatitis C Virus-Infected Egyptian Patients. Biomed Res Int 2020:8879758 (2020). PubMed: 33381596