Mouse p21 ELISA Kit (CDKN1A) (ab205576)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.7 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse p21 ELISA Kit (CDKN1A)
p21 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Cell extract 5 3.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Cell extract 3 7.8% -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.7 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse p21 ELISA Kit (CDKN1A) (ab205576) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p21 (CDKN1A) protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse p21 (CDKN1A) with 2.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
特記事項
p21 binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. p21 functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, p21 inhibits the kinase activity of the cyclin D-CDK4 complex. p21 may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse p21 Capture Antibody 1 x 600µl 10X Mouse p21 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse p21 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
-
機能
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. -
組織特異性
Expressed in all adult human tissues, with 5-fold lower levels observed in the brain. -
配列類似性
Belongs to the CDI family. -
ドメイン
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. -
翻訳後修飾
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
別名
- CAP20
- CDK-interacting protein 1
- CDKI
see all -
参照データベース
- Entrez Gene: 12575 Mouse
- SwissProt: P39689 Mouse
- Unigene: 195663 Mouse
関連製品
-
Alternative Versions
画像
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of p21 were measured in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted NIH/3T3 cell extract samples were at 80 µg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per µg of total protein (mean +/- SD, n=2).
-
NIH/3T3 cells were cultured in the absence or presence of 1 µM camptothecin for 18 hours. Raw 264.7 cells were cultured in the absence of camptothecin. The cell extracts were prepared. The concentrations of p21 were measured in the diluted cell extracts in duplicates, interpolated from the p21 standard curves and corrected for sample dilution. Note that 1X Diluted untreated NIH/3T3 cell extract samples were at 44 µg/mL. Note that 1X Diluted camptothecin treated NIH/3T3 cell extract samples were at 11 µg/mL. Note that 1X Diluted untreated Raw 264.7 cell extract samples were at 6 µg/mL. The interpolated, dilution factor-corrected values are plotted in pg of p21 per µg of total protein (mean +/- SD, n=2).
-
To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab205576 は 1 報の論文で使用されています。
- Kumar VHS et al. Adaptive immune responses are altered in adult mice following neonatal hyperoxia. Physiol Rep 6:N/A (2018). Mouse . PubMed: 29368801