Alexa Fluor® 488 Anti-Nup153 抗体 [QE5] (ab204250)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [QE5] to Nup153
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-Nup153 antibody [QE5]
Nup153 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Mouse monoclonal [QE5] to Nup153 -
由来種
Mouse -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Full length protein corresponding to Rat Nup153. (Rat liver nuclear envelope proteins).
Database link: P49791 -
ポジティブ・コントロール
- ICC/IF: HepG2 cells.
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特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
ab204250 recognises NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. -
ポリ/モノ
モノクローナル -
クローン名
QE5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab204250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
|
特記事項 |
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ICC/IF
1/100. |
ターゲット情報
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機能
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
配列類似性
Contains 4 RanBP2-type zinc fingers. -
ドメイン
Contains F-X-F-G repeats. -
細胞内局在
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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参照データベース
- Entrez Gene: 9972 Human
- Entrez Gene: 218210 Mouse
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
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別名
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
画像
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ab204250 staining Nup153 in HepG2 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204250 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab204250 は論文での使用が確認できていません。