HRP Anti-CNPase 抗体 [11-5B] (ab201678)
Key features and details
- HRP Mouse monoclonal [11-5B] to CNPase
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-CNPase antibody [11-5B]
CNPase 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [11-5B] to CNPase -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Rabbit, Cow, Dog, Pig, Rhesus monkey非交差種: Chicken, Guinea pig -
免疫原
Full length native protein (purified) corresponding to Human CNPase.
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ポジティブ・コントロール
- WB: Human, Mouse and Rat Spinal Cord and Brain tissue lysates. IHC-P: FFPE human normal cerebral cortex tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Batches contain 0.4M arginine. -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
11-5B -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201678の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. -
細胞内局在
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 280752 Cow
- Entrez Gene: 607694 Dog
- Entrez Gene: 1267 Human
- Entrez Gene: 12799 Mouse
- Entrez Gene: 493772 Pig
- Entrez Gene: 25275 Rat
- Entrez Gene: 641337 Rhesus monkey
- Omim: 123830 Human
see all -
別名
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
see all
画像
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All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CNPase knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 minutesab201678 was shown to specifically react with CNPase in wild-type HAP1 cells as signal was lost in CNPase knockout cells. Wild-type and CNPase knockout samples were subjected to SDS-PAGE. Ab201678 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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IHC image of CNPase staining in a section of formalin-fixed paraffin-embedded human normal cerebral cortex*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab201678 at 1/100 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : HRP Anti-CNPase antibody [11-5B] (ab201678) at 1/5000 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Brain (Rat) Tissue Lysate
Lane 6 : Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 6 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201678 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab201678 は論文での使用が確認できていません。