Rat PAI1 ELISA Kit (SERPINE1) (ab198509)
Key features and details
- Sensitivity: 0.079 ng/ml
- Range: 0.1 ng/ml - 50 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Rat
製品の概要
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製品名
Rat PAI1 ELISA Kit (SERPINE1)
PAI1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Known Conc 20 0.957ng/ml 0.088 9.24% Known Conc 20 2.25ng/ml 0.134 5.97% Known Conc 20 15.6ng/ml 1.01 6.48% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Known Conc 10 0.926ng/ml 0.113 12.2% Known Conc 10 1.4ng/ml 0.096 6.86% Known Conc 10 9.17ng/ml 0.858 9.36% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Quantitative -
検出感度
0.079 ng/ml -
検出範囲
0.1 ng/ml - 50 ng/ml -
添加回収試験
104 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Spike 104 97% - 108% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat
非交差種: Sheep -
製品の概要
Abcam’s PAI1 (SERPINE1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of active PAI1 (SERPINE1) in rat plasma and serum.
Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate and will not be detected. Unbound PAI1 samples are washed away and an anti-PAI1 primary antibody is added. Excess primary antibody is washed away and bound antibody, which is proportional to the original active PAI1 present in the samples, is then bound by the horseradish peroxidase secondary antibody. Following an additional washing step, TMB is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-rabbit horseradish peroxidase secondary antibody 1 vial Anti-rat PAI-1 primary antibody 1 vial Rat PAI-1 activity standard 1 vial TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 x 96 tests -
研究分野
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機能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
組織特異性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
関連疾患
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
参照データベース
- Entrez Gene: 24617 Rat
- SwissProt: P20961 Rat
- Unigene: 29367 Rat
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab198509 は 3 報の論文で使用されています。
- Wang C et al. Vepoloxamer Enhances Fibrinolysis of tPA (Tissue-Type Plasminogen Activator) on Acute Ischemic Stroke. Stroke 50:3600-3608 (2019). PubMed: 31587657
- Grasa-López A et al. Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity. Mar Drugs 14:N/A (2016). Rat . PubMed: 27527189
- Jiang H et al. Plasminogen Activator Inhibitor-1 in depression: Results from Animal and Clinical Studies. Sci Rep 6:30464 (2016). Rat . PubMed: 27456456