HRP Anti-GSK3 beta 抗体 [Y174] (ab196911)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [Y174] to GSK3 beta
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-GSK3 beta antibody [Y174]
GSK3 beta 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [Y174] to GSK3 beta -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A431 whole cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y174 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-GSK3 beta antibody [Y174] - BSA and Azide free (ab183177)
- Alexa Fluor® 647 Anti-GSK3 beta antibody [Y174] (ab196910)
- Alexa Fluor® 488 Anti-GSK3 beta antibody [Y174] (ab197236)
- Alexa Fluor® 594 Anti-GSK3 beta antibody [Y174] (ab201737)
- Alexa Fluor® 555 Anti-GSK3 beta antibody [Y174] (ab201738)
- PE Anti-GSK3 beta antibody [Y174] (ab210619)
- Anti-GSK3 beta antibody [Y174] (ab32391)
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196911の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa).
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特記事項 |
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WB
1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 46 kDa). |
ターゲット情報
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機能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
組織特異性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2932 Human
- Entrez Gene: 56637 Mouse
- Omim: 605004 Human
- SwissProt: P49841 Human
- SwissProt: Q9WV60 Mouse
- Unigene: 445733 Human
- Unigene: 394930 Mouse
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別名
- Glycogen Synthase Kinase 3 Beta antibody
- Glycogen synthase kinase-3 beta antibody
- GSK 3 beta antibody
see all
画像
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All lanes : HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GSK3B knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 20 minutesab196911 was shown to recognize GSK3 beta in wild-type HAP1 cells as signal was lost at the expected MW in GSK3B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GSK3B knockout samples were subjected to SDS-PAGE. Ab196911 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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HRP Anti-GSK3 beta antibody [Y174] (ab196911) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196911 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab196911 は論文での使用が確認できていません。