HRP Anti-NQO1 抗体 [A180] (ab196629)
Key features and details
- HRP Mouse monoclonal [A180] to NQO1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
HRP Anti-NQO1 antibody [A180]
NQO1 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [A180] to NQO1 -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Human NQO1.
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ポジティブ・コントロール
- WB: Human kidney tissue lysate. IHC-P: human breast adenocarcinoma tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
A180 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196629の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (2) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
配列類似性
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: Q64669 Mouse
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
別名
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
画像
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All lanes : HRP Anti-NQO1 antibody [A180] (ab196629) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : NQO1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 30 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesab196629 was shown to recognize NQO1 in wild-type HAP1 cells as signal was lost at the expected MW in NQO1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. Ab196629 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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IHC image of NQO1 staining in a section of formalin-fixed paraffin-embedded human breast adenocarcinoma*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196629 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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HRP Anti-NQO1 antibody [A180] (ab196629) at 1/5000 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196629 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab196629 は論文での使用が確認できていません。