Anti-Nkx3.1 抗体 [EPR16653] (ab196020)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16653] to Nkx3.1
- Suitable for: IHC-P, WB, mIHC
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Nkx3.1 antibody [EPR16653]
Nkx3.1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16653] to Nkx3.1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, mIHCmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human prostate lysate and LNCaP cell lysate. IHC-P: Human prostate hyperplasia tissue. mIHC: Human prostate gland tissues.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16653 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab196020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
|
|
mIHC |
1/2000.
|
特記事項 |
---|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
mIHC
1/2000. |
ターゲット情報
-
機能
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. -
組織特異性
Highly expressed in the prostate and, at a lower level, in the testis. -
配列類似性
Belongs to the NK-3 homeobox family.
Contains 1 homeobox DNA-binding domain. -
翻訳後修飾
Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 4824 Human
- Omim: 602041 Human
- SwissProt: Q99801 Human
- Unigene: 55999 Human
-
別名
- BAPX 2 antibody
- BAPX2 antibody
- Homeobox protein NK-3 homolog A antibody
see all
画像
-
All lanes : Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Lane 2, PC-3 (Human prostate cancer cell line) is acting as a negative control for Nkx3.1. The 32kDa band may represent the glycosylated form.
-
Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Prostate Specific Antigen (ab76113, red; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Nkx3.1 (ab196020, gray; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on cytoplasm of luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-p63 stained on nucleus of luminal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab76113 (1/2000), ab236216 (1/400) and ab196020 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
-
Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human prostate hyperplasia tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution + Human prostate lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human testis tissue acting as a negative control for Nkx3.1. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (3)
ab196020 は 3 報の論文で使用されています。
- Luo A et al. KLF5-induced miR-487a augments the progression of osteosarcoma cells by targeting NKX3-1 in vitro. Oncol Lett 24:258 (2022). PubMed: 35765275
- Wu T et al. Targeting HIC1/TGF-β axis-shaped prostate cancer microenvironment restrains its progression. Cell Death Dis 13:624 (2022). PubMed: 35853880
- Leibold J et al. Somatic Tissue Engineering in Mouse Models Reveals an Actionable Role for WNT Pathway Alterations in Prostate Cancer Metastasis. Cancer Discov 10:1038-1057 (2020). PubMed: 32376773