Human beta Nerve Growth Factor ELISA Kit (ab193760)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6 pg/ml
- Range: 27.3 pg/ml - 1750 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human beta Nerve Growth Factor ELISA Kit
Beta nerve growth factor キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5 3.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 3 6.7% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6 pg/ml -
検出範囲
27.3 pg/ml - 1750 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96 95% - 99% Urine 96 94% - 100% Serum 94 92% - 95% Hep Plasma 93 92% - 95% EDTA Plasma 88 83% - 92% Cit plasma 82 76% - 88% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human beta Nerve Growth Factor ELISA Kit (ab193760) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of beta Nerve Growth Factor protein in edta plasma, hep plasma, urine, serum, cell culture supernatant, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human beta Nerve Growth Factor with 6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Beta Nerve Growth Factor is involved in the development of maintenance of the sympathetic and sensory nervous systems. As a ligand for NTRK1 and NGFR receptors, it’s involved in cell signaling cascades that regulate neuronal proliferation, differentiation, and survival.
Beta Nerve Growth Factor is also involved in modulation of the immune system and in the trauma response, and has been implicated in a variety of responses including memory and mental health.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human beta NGF Capture Antibody 1 x 600µl 10X Human beta NGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human beta NGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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関連性
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta. -
細胞内局在
Secreted. -
別名
- Beta nerve growth factor
- Beta NGF
- HSAN5
see all -
参照データベース
- Entrez Gene: 4803 Human
- Omim: 162030 Human
- SwissProt: P01138 Human
関連製品
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ELISA kits
画像
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab193760 は 5 報の論文で使用されています。
- Tsai T et al. Changes of Subjective Symptoms and Tear Film Biomarkers following Femto-LASIK. Int J Mol Sci 23:N/A (2022). PubMed: 35886858
- Basabrain MS et al. Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla. Bioengineering (Basel) 9:N/A (2022). PubMed: 36354515
- Veith A et al. Optimized design of a hyperflexible sieve electrode to enhance neurovascular regeneration for a peripheral neural interface. Biomaterials 275:120924 (2021). PubMed: 34147716
- Wu LY et al. The surgical effect on overactive bladder symptoms in women with pelvic organ prolapse. Sci Rep 11:20193 (2021). PubMed: 34642384
- Guo L et al. Topical recombinant human Nerve growth factor (rh-NGF) is neuroprotective to retinal ganglion cells by targeting secondary degeneration. Sci Rep 10:3375 (2020). PubMed: 32099056