Anti-NDUFS2 抗体 [EPR16266] (ab192022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16266] to NDUFS2
- Suitable for: ICC/IF, WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NDUFS2 antibody [EPR16266]
NDUFS2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16266] to NDUFS2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HAP1, HeLa, Jurkat, 293T cell lysate. Mouse brain, kidney and spleen lysate. Rat brain and kidney lysate. C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. IHC-P: Human brain tissue. Rat stomach tissue. IP: 293T cell lysate. ICC/IF: Wild-type HAP1 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16266 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- NIH/3T3 whole cell lysate (ab7179)
- RAW 264.7 whole cell lysate (ab7187)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192022の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.4 µg/ml.
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WB |
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/40.
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特記事項 |
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ICC/IF
Use a concentration of 0.4 µg/ml. |
WB
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/40. |
ターゲット情報
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機能
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. -
関連疾患
Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. -
配列類似性
Belongs to the complex I 49 kDa subunit family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4720 Human
- Entrez Gene: 226646 Mouse
- Entrez Gene: 289218 Rat
- Omim: 602985 Human
- SwissProt: O75306 Human
- SwissProt: Q91WD5 Mouse
- SwissProt: Q641Y2 Rat
- Unigene: 173611 Human
see all -
別名
- CI 49 antibody
- CI 49kD antibody
- CI-49kD antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: NDUFS2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab192022 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab192022 was shown to specifically react with NDUFS2 in wild-type HAP1 cells whilst signal was lost in NDUFS2 knockout cells. Wild-type and NDUFS2 knockout samples were subjected to SDS-PAGE. ab192022 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human brain tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ab192022 staining NDUFS2 in wild-type Hap1 cells (top panel) and NDUFS2 knockout Hap1 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192022 at 0.4μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. -
Lane 1: 293T cell lysate was immunoprecipitated using ab192022 at a 1/40 dilution. Secondary used was anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.
Lane 2: PBS instead of 293T lysates.
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All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
Immunohistochemical analysis of paraffin-embedded rat stomach tissue sections labeling NDUFS2 using ab192022 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-NDUFS2 antibody [EPR16266] (ab192022) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : C6 cell lysate
Lane 7 : RAW264.7 cell lysate
Lane 8 : PC-12 cell lysate
Lane 9 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab192022 は 7 報の論文で使用されています。
- Dai Q et al. Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution. Nat Biotechnol 41:344-354 (2023). PubMed: 36302989
- Bartolomeo CS et al. SARS-CoV-2 infection and replication kinetics in different human cell types: The role of autophagy, cellular metabolism and ACE2 expression. Life Sci 308:120930 (2022). PubMed: 36075471
- Bennett CF et al. Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly. Nat Chem Biol 17:703-710 (2021). PubMed: 33723432
- Niu F et al. Protein profiling identified mitochondrial dysfunction and synaptic abnormalities after dexamethasone intervention in rats with traumatic brain injury. Neural Regen Res 16:2438-2445 (2021). PubMed: 33907032
- Ruan QT et al. A Mutation in Hnrnph1 That Decreases Methamphetamine-Induced Reinforcement, Reward, and Dopamine Release and Increases Synaptosomal hnRNP H and Mitochondrial Proteins. J Neurosci 40:107-130 (2020). PubMed: 31704785
- Jing YY et al. Epigenetic regulation of the Warburg effect by H2B monoubiquitination. Cell Death Differ 27:1660-1676 (2020). PubMed: 31685978
- Gregg T et al. Obesity-dependent CDK1 signaling stimulates mitochondrial respiration at complex I in pancreatic ß-cells. J Biol Chem 294:4656-4666 (2019). PubMed: 30700550