Human RAGE ELISA Kit (ab190807)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.4 pg/ml
- Range: 46.87 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human RAGE ELISA Kit
RAGE キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% EDTA plasma 5 1.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% EDTA plasma 3 7.6% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.4 pg/ml -
検出範囲
46.87 pg/ml - 3000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 94.9 91.8% - 97.8% Cell culture media 98.6 96.8% - 101.1% Hep Plasma 96.7 95.5% - 98% EDTA Plasma 107.2 101.7% - 116.9% Cit plasma 86.6 78% - 92.6% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
非交差種: Goat, Cow, Pig -
製品の概要
Human RAGE ELISA Kit (ab190807) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RAGE protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human RAGE with 2.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
特記事項
RAGE mediates interactions of advanced glycosylation end products (AGE). These are non-enzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. RAGE acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. RAGE interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key pro-inflammatory mediators. RAGE may be a receptor for amyloid beta peptide. RAGE contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intra-neuronal space.
-
試験プラットフォーム
Microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human RAGE Capture Antibody 1 x 600µl 10X Human RAGE Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human RAGE Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
-
機能
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. -
組織特異性
Endothelial cells. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
別名
- Advanced glycosylation end product-specific receptor
- Ager
- DAMA 358M23.4
see all -
参照データベース
- Entrez Gene: 177 Human
- Omim: 600214 Human
- SwissProt: Q15109 Human
- Unigene: 534342 Human
関連製品
-
ELISA kits
画像
-
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
Background-subtracted data values (mean +/- SD) are graphed.
-
The concentrations of RAGE were measured in duplicates, interpolated from the RAGE standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 22% and plasma (heparin) 22%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RAGE concentration was determined to be 1,088 pg/mL in serum and 679 pg/mL in plasma (heparin).
-
The concentrations of RAGE were measured in duplicates, interpolated from the RAGE standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RAGE concentration was determined to be 592 pg/mL in plasma (citrate) and 797 pg/mL in plasma (EDTA).
-
4.5X diluted serum samples from 10 apparently healthy male donors were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in pg of RAGE per mL of serum (mean +/- SD, n=3). The mean of RAGE concentration of these serum samples was determined to be 604 pg /mL with a range of 220 – 1276 pg /mL.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab190807 は 2 報の論文で使用されています。
- Li H et al. Serum levels of laminin and von Willebrand factor in COVID-19 survivors 6 months after discharge. Int J Infect Dis 115:134-141 (2022). PubMed: 34843955
- Niepolski L et al. Circulating vascular endothelial growth factor receptor 2 levels and their association with lipid abnormalities in patients on hemodialysis. Biomed Rep 14:37 (2021). PubMed: 33692900