Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Suitable for: SDS-PAGE, Agglutination, WB
製品の詳細
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製品名
Recombinant Influenza A Virus Hemagglutinin H5 protein -
精製度
> 80 % SDS-PAGE. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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配列
mekivllfai vslvksdqic igyhannste qvdtimeknv tvthaqdile kkhngklcdl dgvkplilrd csvagwllgn pmcdefinvp ewsyivekan pvndlcypgd fndyeelkhl lsrinhfeki qiipksswss heaslgvssa cpyqgkssff rnvvwlikkn styptikrsy nntnqedllv lwgihhpnda aeqtklyqnp ttyisvgtst lnqrlvpria trskvngqsg rmeffwtilk pndainfesn gnfiapeyay kivkkgdsti mkseleygnc ntkcqtpmga inssmpfhni hpltigecpk yvksnrlvla tglrnspqre rrrkkrglfg aiagfieggw qgmvdgwygy hhsneqgsgy aadkestqka idgvtnkvns iidkmntqfe avgrefnnle rrienlnkkm edgfldvwty naellvlmen ertldfhdsn vknlydkvrl qlrdnakelg ngcfefyhkc dnecmesvrn gtydypqyse earlkreeis gvklesigiy qilsiystva sslalaimva glslwmcsng slqcrici -
予測される分子量
64 kDa -
領域
1 to 567 -
配列の追加情報
Influenza Hemagglutinin Full Length Glycoprotein (rHA GP) from virus strain A/Vietnam/1203/2004 (H5N1). The theoretical molecular weight of the protein is ~64 kDa, without glycosylation.
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特性
Our Abpromise guarantee covers the use of ab190125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Agglutination
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 99% Tris buffered saline
0.01% non-ionic detergent
関連情報
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別名
- H5HA
- Hemagglutinin 5
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関連性
Influenza A belongs to the Orthomyxoviridae family, and is a negative sense single-stranded RNA virus which results in respiratory disease. Haemagglutinin is an antigenic glycoprotein which allows viral attachment and entry into the cell. Sixteen subtypes of haemagglutinin (H1-H16) have been described, of which H1, H2 and H3 infect humans. H5 and H7 normally result in avian disease, with some highly pathogenic H5N1 strains causing 100% mortality in poultry. H5N1 has in some cases mutated to infect humans, with 60% mortality. Haemagglutinin is one of the most medically relevant antigens on influenza as it is a target for antiviral drugs and antibodies.
画像
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anti-H5 ferret antiserum at 1/20 dilution +
Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125) at 0.2 µg
Secondary
an anti-ferret AP conjugate followed by substrate. -
SDS-PAGE analysis of 1.5 μg of ab190125 protein under reducing conditions.
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Hemagglutination with turkey red blood cells: ab190125 titer 1:8192
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab190125 は 4 報の論文で使用されています。
- Castellanos-Huerta I et al. Transformation of Dunaliella salina by Agrobacterium tumefaciens for the Expression of the Hemagglutinin of Avian Influenza Virus H5. Microorganisms 10:N/A (2022). PubMed: 35208815
- Bizyaeva AA et al. The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus. Int J Mol Sci 22:N/A (2021). PubMed: 33673708
- Nakakita SI et al. Preparation and Detection of Glycan-Binding Activity of Influenza Virus. Methods Mol Biol 2132:567-583 (2020). PubMed: 32306357
- Novoseltseva AA et al. Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses. Biomolecules 10:N/A (2020). PubMed: 31936820