Human PDGF BB ELISA Kit (ab184860)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.9 pg/ml
- Range: 11.7 pg/ml - 750 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PDGF BB ELISA Kit
PDGF B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Human Serum 8 3.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Human Serum 3 5.3% -
サンプルの種類
Serum, Plasma, Cell culture media -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.9 pg/ml -
検出範囲
11.7 pg/ml - 750 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 95 85% - 100% Cell culture media 104 101% - 106% Hep Plasma 104 92% - 114% EDTA Plasma 99 91% - 107% Cit plasma 109 100% - 114% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human PDGF BB ELISA kit (ab184860) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF BB protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human PDGF BB with 2.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
特記事項
Platelet derived growth factor B chain plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 10X Human PDGF BB Capture Antibody 1 x 600µl 10 x 600µl 10X Human PDGF BB Detector Antibody 1 x 600µl 10 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 4BI 1 x 6ml 10 x 6ml Human PDGF BB Lyophilized Recombinant Protein 2 vials 20 vials Plate Seals 1 unit 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究分野
- Information by UniProt
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別名
- Becaplermin
- c sis
- FLJ12858
see all -
参照データベース
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
関連製品
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ELISA kits
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The PDGF BB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Background subtracted data from duplicate measurements are plotted.
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Mean human PDGF-BB is 145 pg/mL and values fall within expected normal reference ranges (WHO).
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Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours aliquoted and assayed for endogenous PDGF-BB levels.
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Standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed. In addition, PDGF-A was prepared at 15 ng/mL and assayed for cross reactivity. No cross reactivity or interference was observed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab184860 は 4 報の論文で使用されています。
- Yang Y & Min Z Effect of long non-coding RNA AK021443 on promoting hepatic fibrosis in vitro. Mol Med Rep 23:N/A (2021). PubMed: 33495828
- Mechelinck M et al. Serum from Patients with Severe Alcoholic Liver Cirrhosis Inhibits Proliferation and Migration of Human Coronary Artery Smooth Muscle Cells. J Clin Med 10:N/A (2021). PubMed: 34884173
- Wakayama T et al. Quality comparison between two different types of platelet-rich plasma for knee osteoarthritis. Regen Med Res 8:3 (2020). PubMed: 33287956
- Pirklbauer M et al. Unraveling reno-protective effects of SGLT2 inhibition in human proximal tubular cells. Am J Physiol Renal Physiol 316:F449-F462 (2019). PubMed: 30539648