Anti-ATF7 抗体 [EPR13556] (ab183507)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13556] to ATF7
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ATF7 antibody [EPR13556]
ATF7 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13556] to ATF7 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Raji, C6, Raw 264.7 and PC-12 cell lysates
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13556 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 53 kDa.
|
特記事項 |
---|
WB
1/1000. Predicted molecular weight: 53 kDa. |
ターゲット情報
-
機能
Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter. -
組織特異性
Expressed in heart, lung and skeletal muscle. Isoform 4 is expressed in various tissues including heart, brain, placenta, lung and skeletal muscle. Highest levels in skeletal muscle. Lowest in lung and placenta. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 C2H2-type zinc finger. -
翻訳後修飾
On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase. -
細胞内局在
Nucleus. Nucleus > nucleoplasm. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery. - Information by UniProt
-
参照データベース
- Entrez Gene: 11016 Human
- Entrez Gene: 223922 Mouse
- Entrez Gene: 315333 Rat
- Omim: 606371 Human
- SwissProt: P17544 Human
- SwissProt: Q8R0S1 Mouse
- Unigene: 12286 Human
- Unigene: 208714 Mouse
see all -
別名
- 1110012F10Rik antibody
- 9430065F09Rik antibody
- Activating transcription factor 7 antibody
see all
画像
-
Anti-ATF7 antibody [EPR13556] (ab183507) at 1/1000 dilution + Raji cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
All lanes : Anti-ATF7 antibody [EPR13556] (ab183507) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (3)
ab183507 は 3 報の論文で使用されています。
- He M et al. LncRNA PCNAP1 Promotes Hepatoma Cell Proliferation through Targeting miR-340-5p and is Associated with Patient Survival. J Oncol 2021:6627173 (2021). PubMed: 34007276
- Liu W et al. VAV2 is required for DNA repair and implicated in cancer radiotherapy resistance. Signal Transduct Target Ther 6:322 (2021). PubMed: 34462423
- Verma N et al. The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3. J Biol Chem 295:5984-5994 (2020). PubMed: 32184357