Anti-HA tag 抗体 [EPR4095] (ab182009)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4095] to HA tag
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
-
製品名
Anti-HA tag antibody [EPR4095]
HA tag 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4095] to HA tag -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Multi-tag fusion protein. HA-tagged Human Glutaminase C overexpression lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4095 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182009の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000.
|
特記事項 |
---|
WB
1/1000 - 1/10000. |
ターゲット情報
-
関連性
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. -
別名
- HA epitope tag antibody
- HA1 antibody
- HA2 antibody
see all
画像
-
Anti-HA tag antibody [EPR4095] (ab182009) at 1/10000 dilution (Purified) + HA-tagged Human Glutaminase C overexpression lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Anti-HA tag antibody [EPR4095] (ab182009) at 1/5000 dilution + multi-tag fusion protein, 1 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutionBlocking/ Dilution buffer: 5% NFDM /TBST.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (5)
ab182009 は 5 報の論文で使用されています。
- Lu Y et al. Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B. Front Cell Dev Biol 10:833396 (2022). PubMed: 35669517
- Guo Q et al. ZEB2, interacting with MDM2, contributes to the dysfuntion of brain microvascular endothelial cells and brain injury after intracerebral hemorrhage. Cell Cycle 20:1692-1707 (2021). PubMed: 34334113
- Barz S et al. Atg1 kinase regulates autophagosome-vacuole fusion by controlling SNARE bundling. EMBO Rep 21:e51869 (2020). PubMed: 33274589
- Hollenstein DM et al. Vac8 spatially confines autophagosome formation at the vacuole in S. cerevisiae. J Cell Sci 132:N/A (2019). PubMed: 31649143
- Driesbaugh KH et al. Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin. J Biol Chem 290:3529-41 (2015). Human . PubMed: 25519908