Anti-TOMM22/TOM22 抗体 [EPR13505] - N-terminal (ab179826)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13505] to TOMM22/TOM22 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal
TOMM22/TOM22 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13505] to TOMM22/TOM22 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human TOMM22/TOM22 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NS69 -
ポジティブ・コントロール
- HeLa, K562, Raji and 293T cell lysates. Kidney and liver tissue. Raji and 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13505 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179826の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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細胞内局在
Mitochondrion outer membrane; Single-pass membrane protein -
参照データベース
- Entrez Gene: 56993 Human
- Omim: 607046 Human
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別名
- 1C9 2 antibody
- hTom 22 antibody
- hTom22 antibody
see all
画像
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All lanes : Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Immunofluorescent analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/10 (red) or a rabbit IgG (negative) (green).
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Western blot analysis on immunoprecipitation pellet from Raji cell lysate using ab179826 at 1/10 dilution for IP.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab179826 は 3 報の論文で使用されています。
- Furihata T et al. Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Commun Biol 4:138 (2021). PubMed: 33514783
- Alowaidi F et al. Investigating the role of CRIPTO-1 (TDGF-1) in glioblastoma multiforme U87 cell line. J Cell Biochem N/A:N/A (2018). PubMed: 30426531
- Pollara J et al. Circulating mitochondria in deceased organ donors are associated with immune activation and early allograft dysfunction. JCI Insight 3:N/A (2018). PubMed: 30089724