Goat Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175710)
Key features and details
- Goat Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed
- Conjugation: Alexa Fluor® 568. Ex: 578nm, Em: 603nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA
製品の概要
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製品名
Goat Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Rat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chain common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISAmore details -
吸着処理血清
Chicken, Cow, Human, Mouse, Rabbit, Sheep more details -
免疫原
The details of the immunogen for this antibody are not available.
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標識
Alexa Fluor® 568. Ex: 578nm, Em: 603nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was cross adsorbed using bovine, chicken, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
1/2000.
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ICC/IF |
1/200 - 1/1000.
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
1/2000. |
ICC/IF
1/200 - 1/1000. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
画像
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ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175710 Alexa Fluor® 568 Goat anti-Rat IgG (H&L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
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HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175710 Alexa Fluor® 568 Goat anti-Rat IgG (H&L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab175710 は 8 報の論文で使用されています。
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- Szebényi K et al. Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology. Nat Neurosci 24:1542-1554 (2021). PubMed: 34675437
- Fried S et al. Extrahepatic cholangiocyte obstruction is mediated by decreased glutathione, Wnt and Notch signaling pathways in a toxic model of biliary atresia. Sci Rep 10:7599 (2020). PubMed: 32371929
- Yoshizawa K et al. Uncoupling of DNA Replication and Centrosome Duplication Cycles Is a Primary Cause of Haploid Instability in Mammalian Somatic Cells. Front Cell Dev Biol 8:721 (2020). PubMed: 32850837
- Fujita H et al. Premature aging syndrome showing random chromosome number instabilities with CDC20 mutation. Aging Cell 19:e13251 (2020). PubMed: 33094908
- Inoue A et al. Protocol for BATTLE-1EX: A High-Resolution Imaging Method to Visualize Whole Synaptic Structures and their Components in the Nervous System. STAR Protoc 1:100166 (2020). PubMed: 33377060
- Kurosaki F et al. Optimization of adeno-associated virus vector-mediated gene transfer to the respiratory tract. Gene Ther 24:290-297 (2017). PubMed: 28346434
- Bloch D et al. Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth. Plant Physiol 172:980-1002 (2016). PubMed: 27516531