Anti-Polyethylene glycol 抗体 [PEG-B-47] - BSA and Azide free (ab170969)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [PEG-B-47] to Polyethylene glycol - BSA and Azide free
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
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製品名
Anti-Polyethylene glycol antibody [PEG-B-47] - BSA and Azide free
Polyethylene glycol 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [PEG-B-47] to Polyethylene glycol - BSA and Azide free -
由来種
Rabbit -
特異性
This antibody recognizes the terminal methoxy group of the PEG molecule. This antibody does not cross react with non-specific targets in blood or serum. Both free form of PEG (unconjugated version) and conjugated PEG were tested by sandwich ELISA using ab51257; results showed that ab51257 only detects conjugated forms of PEG.
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アプリケーション
適用あり: ELISA, WB, IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Mouse kidney, spleen, muscle and liver tissue - (animals injected with a PEGylated protein).
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特記事項
ab170969 is the carrier-free version of ab51257.
If you have any questions on our PEG products - please visit our Polyethylene glycol (PEG) FAQs page.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 2.41 x 10 -9 M Learn more about KD -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
PEG-B-47 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170969の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
This antibody only works on the tissues when the animals are injected with a PEGylated protein. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. This antibody only works on the tissues when the animals are injected with a PEGylated protein. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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別名
- PEG antibody
画像
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ELISA assay using ab51257 to detect different forms of PEG. PEG40000-BSA is a 40 kDa PEG molecule attached to BSA. PEG5000-BSA is a 5 kDa linear PEG molecule attached to BSA.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).
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This antibody affinity data was generated using the same anti-PEG antibody clone, PEG-B-47, in a different buffer formulation (ab51257).
Method:Protein A sensor + Antigen (PEG 5K-BSA at 0.3µg/ml)+ antibody (ab51257 at 0.0312, 0.0625, 0.125, 0.25, 0.5, 1 µg/m).
Results:
Sample ID: PEG-5K-BSA
KD (M): 2.41E-09
kon(1/Ms): 2.67E+04
kon Error: 6.81E+02
kdis(1/s): 6.43E-05
kdis Error: 2.92E-06
Full X^2: 0.018444
Full R^2: 0.982059
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Comparison of ab51257 and Vendor A mouse MAb in Direct ELISA assy.
Goat anti-rabbit IgG-AP used for anti-PEG-47 detection; goat anti-mouse IgM-AP used for Vendor A MAb detection.
Fig 1a. Direct ELISA using 1 ug/mL of PEG-GCSF.
Fig 1b. Direct ELISA using 1 ug/mL of PEG-IFN.
Comparison to other anti-PEG: In both direct and sandwich ELISA assays, ab51257 shows greater affinity and accuracy than other anti-PEG antibodies when determining the concentration of PEG or PEG-modified proteins. Results were similar whether detecting PEG itself or PEG-modified targets.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).
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Comparison of sandwich ELISA using RabMAb/RabMAb (ab51257/ab51257) and MAb/RabMAb (Vendor A/ab51257) for capture/detection.
Ab51257/ab51257*: Plate coated with 5 ug/mL of #47; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)
Vendor A/ ab51257*: Plates coated with 100 ug/mL of Vendor A Mouse MAb; 5 ug/mL of #47 used for detection (*Anti-PEG 47 biotin labeled)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).
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Comparison of 10 ug/well of activated linear (PEG5K) and branched (PEG40K) PEG using 5 ug/ml of ab51257 in Direct ELISA assay.
Accuracy: By detecting the methoxy group of the PEG molecule itself, ab51257 is useful in measuring the pharmacokinetics of PEG-modified molecules in vivo. Data indicate that ab51257 detects various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Ab51257 does not cross react with non-specific targets in blood or serum.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51257).
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This data was developed using ab51257, the same antibody clone in a different buffer formulation.
ELISA Graph generated using purified ab51257 at 1μg/mL.
Antigen concentration range:
PEG5000 and PEG20000: 0.0078 - 0.5 μg/mL
ADI-PEG: 0.0156 - 1 μg/mL
PEG-BSA: 0.0039 - 0.25μg/mL
PEG-IFN-α-2b0.0156 - 1 μg/mLSecondary antibody was an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) at 1/2500.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab170969 は 1 報の論文で使用されています。
- Petrou CL et al. Clickable decellularized extracellular matrix as a new tool for building hybrid-hydrogels to model chronic fibrotic diseases in vitro. J Mater Chem B 8:6814-6826 (2020). PubMed: 32343292