Anti-gamma Tubulin 抗体 - Centrosome Marker (ab16504)
Key features and details
- Rabbit polyclonal to gamma Tubulin - Centrosome Marker
- Suitable for: ICC/IF, WB
- Reacts with: Human, Zebrafish
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-gamma Tubulin antibody - Centrosome Marker
gamma Tubulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to gamma Tubulin - Centrosome Marker -
由来種
Rabbit -
特異性
Does not react with alpha or beta tubulin. Immunogen sequence found in both gamma tubulin 1 and gamma tubulin 2. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human, Zebrafish
交差が予測される動物種: Mouse, Rat, Dog, Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human gamma Tubulin aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17097) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16504の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (4) |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
Abcam recommends using milk as the blocking agent. |
特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Abcam recommends using milk as the blocking agent. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 403695 Dog
- Entrez Gene: 490962 Dog
- Entrez Gene: 27175 Human
- Entrez Gene: 7283 Human
- Entrez Gene: 103733 Mouse
- Entrez Gene: 103768 Mouse
- Entrez Gene: 252921 Rat
- Entrez Gene: 680991 Rat
see all -
別名
- Gamma 1 tubulin antibody
- Gamma 2 tubulin antibody
- Gamma Tubulin 1 antibody
see all
画像
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ICC/IF image of ab16504 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16504, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 5 : HeLa Nuclear Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lane 6 : A431 Whole Cell Lysate with Human gamma Tubulin peptide (ab17097) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 48 kDaLane 1 - 6 : gamma Tubulin antibody - Centrosome Marker (ab11317) at 1 ug/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Cell Lysate
Lane 3 : A431 Whole Cell Lysate
Lane 4 : HeLa Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 5 : HeLa Nuclear Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Lane 6 : A431 Whole Cell Lysate with gamma Tubulin peptide (38-53) (ab17097)
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lysates at 20 ug.
Blocking peptide at 1 ug/ml.
Performed under reducing conditions.
Observed band size : 48kD -
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504 (1/300). ab16504 staining is localized to the centrosome (red). The cells were counterstained with DAPI (blue). 100x magnification. The cells were blocked with 5% fetal bovine serum.
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SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16504. The antibody clearly labels the centrosome (red). The cells were counterstained with DAPI (blue). The cells were blocked in 5% BSA.
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ICC/IF image of ab16504 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16504, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The cause of the background staining is uncertain, although a cytokeratin exisits of a similar molecular weight and amino acid sequence to that of the immunogen used to raise the antibody. -
All lanes : Anti-gamma Tubulin antibody - Centrosome Marker (ab16504) at 1 µg/ml
Lane 1 : Molecular Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish liver homogenate at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) whole cell lysate at 20 µg
Secondary
Lane 1 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Lanes 2-4 : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (25)
ab16504 は 25 報の論文で使用されています。
- Speight P et al. Myocardin-related transcription factor and serum response factor regulate cilium turnover by both transcriptional and local mechanisms. iScience 24:102739 (2021). PubMed: 34278253
- Ghelli Luserna Di Rorà A et al. Exploring the ATR-CHK1 pathway in the response of doxorubicin-induced DNA damages in acute lymphoblastic leukemia cells. Cell Biol Toxicol N/A:N/A (2021). PubMed: 34519926
- Ma Z et al. Primary cilia-dependent signaling is involved in regulating mesenchymal stem cell proliferation and pluripotency maintenance. J Mol Histol 51:241-250 (2020). PubMed: 32399704
- Martella C et al. Human T-Cell Lymphotropic Virus Type 1 Transactivator Tax Exploits the XPB Subunit of TFIIH during Viral Transcription. J Virol 94:N/A (2020). PubMed: 32024775
- Ishikawa KI et al. p150glued deficiency impairs effective fusion between autophagosomes and lysosomes due to their redistribution to the cell periphery. Neurosci Lett 690:181-187 (2019). PubMed: 30366015