Granzyme B Activity Assay Kit (Fluorometric) (ab157403)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
製品の概要
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製品名
Granzyme B Activity Assay Kit (Fluorometric)
Granzyme B キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell Lysate -
アッセイタイプ
Enzyme activity (quantitative) -
種交差性
交差種: Mammals, Other species -
製品の概要
In Abcam's Granzyme B Activity Assay Kit (Fluorometric), active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K168 Granzyme B Activity Fluorometric Assay Kit. K168-100 is the same size as the 100 test size of ab157403.
Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests AFC Standard 1 x 0.1ml Granzyme B Assay Buffer 1 x 25ml Granzyme B Positive Control 1 vial Granzyme B Substrate 1 x 0.5ml -
研究分野
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機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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別名
- C11
- Cathepsin G like 1
- Cathepsin G-like 1
see all
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab157403 は 1 報の論文で使用されています。
- Rogers OC et al. Targeted delivery of cytotoxic proteins to prostate cancer via conjugation to small molecule urea-based PSMA inhibitors. Sci Rep 11:14925 (2021). PubMed: 34290365