HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control (ab157396)
製品の概要
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製品名
HeLa DNA Damage Whole Cell Lysate Set: UV Treated and Untreated Control -
種交差性
交差種: Human -
製品の概要
UV light is a common source of DNA damage, and can lead to skin cancer and premature aging. Exposure to UV-B and UV-C light leads to the formation of pyrimidine dimers, and to a lesser extent, purine dimers and pyrimidine photophosphates. These dimerized DNA bases are typically removed by the nucleotide excision repair pathway. Failure to repair the damage can induce apoptosis by blocking DNA replication and transcription.
The UV-treated HeLa lysate is designed for use as a western blot positive control when studying UV-induced DNA damage and/or apoptosis. Cells were treated with UV-C light for 1 minute, then cultured for 4 hours before collecting for lysates. Untreated cells were grown under standard cell culture conditions for HeLa cells.
Samples are provided solubilized in an SDS-PAGE loading buffer, supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.
Concentration: HeLa UV-treated lysate, 200 µg at 2.0 mg/mL
HeLa untreated lysate, 200 µg at 2.0 mg/mL -
アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at -80°C. Please refer to protocols. -
内容 2 units Control for UV-Treated HeLa Lysate 1 x 200µg UV-Treated HeLa Lysate 1 x 200µg -
研究分野
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アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
画像
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Lane 1: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396).
All lysates at 20 µg per lane.
Primary antibodies (all lanes): ab136812 Apoptosis Western Blot Cocktail (pro/p17-caspase 3, cleaved-PARP, muscle actin) 1:250 dilution.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution and Goat polyclonal to Rabbit IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band sizes: 89, 42, 32, 17 kDa
Observed band sizes: 89, 42, 32 kDa -
Lane 1: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396)
All lysates at 20 µg per lane.
Primary antibody (all lanes): ab133981 Anti-Caspase 9 antibody at 2 µg/mL.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band size: 35 kDa
Observed band size: 25 kDa -
Lanes 1, 4: MW marker
Lane 2: Untreated HeLa lysate (ab157396)
Lane 3: UV-treated HeLa lysate (ab157396).
All lysates at 20 µg per lane.
Primary antibodies:
Lanes 1-3: ab131385 Apoptosis and DNA Damage Western Blot Cocktail (cleaved PARP, GAPDH, H2A.X pSer139) 1:250 dilution
Lanes 4-6: H2A.X pSer139 antibody.
Secondary antibodies (all lanes): Goat polyclonal to Mouse IgG - HRP at 1:5000 dilution.
Developed using the ECL method.
Predicted band sizes: 15, 36, 89 kDa
Other bands observed (from H2A.X pSer139 antibody, identities unknown): 25, 30 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157396 は論文での使用が確認できていません。