Anti-PPP2R1A 抗体 (ab154551)
Key features and details
- Rabbit polyclonal to PPP2R1A
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPP2R1A antibody
PPP2R1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPP2R1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Zebrafish, Xenopus tropicalis -
免疫原
Recombinant fragment corresponding to a region within amino acids 308-589 of PPP2R1A (Uniprot ID P30153)
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ポジティブ・コントロール
- HeLa, NIH-3T3, JC, BCL-1 whole cell lysate; HeLa cells; U87 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154551の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
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ICC/IF | (1) |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. -
配列類似性
Belongs to the phosphatase 2A regulatory subunit A family.
Contains 15 HEAT repeats. -
ドメイン
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure. -
細胞内局在
Cytoplasm. Chromosome > centromere. Centromeric localization requires the presence of BUB1. - Information by UniProt
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参照データベース
- Entrez Gene: 535321 Cow
- Entrez Gene: 5518 Human
- Entrez Gene: 51792 Mouse
- Entrez Gene: 397088 Pig
- Entrez Gene: 117281 Rat
- Entrez Gene: 406685 Zebrafish
- Omim: 605983 Human
- SwissProt: Q32PI5 Cow
see all -
別名
- 2AAA_HUMAN antibody
- 6330556D22Rik antibody
- Alpha isoform of regulatory subunit A, protein phosphatase antibody
see all
画像
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Anti-PPP2R1A antibody (ab154551) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
All lanes : Anti-PPP2R1A antibody (ab154551) at 1/5000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PPP2R1A with ab154551 antibody at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded U87 xenograft labeling PPP2R1A with ab154551 antibody at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154551 は 2 報の論文で使用されています。
- Elgenaidi IS & Spiers JP Hypoxia modulates protein phosphatase 2A through HIF-1a dependent and independent mechanisms in human aortic smooth muscle cells and ventricular cardiomyocytes. Br J Pharmacol 176:1745-1763 (2019). PubMed: 30825189
- Lombardi LM et al. An RNA interference screen identifies druggable regulators of MeCP2 stability. Sci Transl Med 9:N/A (2017). PubMed: 28835516