Anti-ATOX1 抗体 [EPR10352] (ab154179)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10352] to ATOX1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ATOX1 antibody [EPR10352]
ATOX1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10352] to ATOX1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, HeLa and 293T cell lysates. IHC-P: Human prostatic hyperplasia tissue IP: HeLa.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10352 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 7 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/100 - 1/500.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 7 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/100 - 1/500. |
ターゲット情報
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機能
Could bind and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the ATX1 family.
Contains 1 HMA domain. - Information by UniProt
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参照データベース
- Entrez Gene: 475 Human
- Omim: 602270 Human
- SwissProt: O00244 Human
- Unigene: 125213 Human
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別名
- ATOX1 antibody
- ATOX1_HUMAN antibody
- ATX1 antibody
see all
画像
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All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ATOX1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.
ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling ATOX1 with ab154179 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : ATOX1 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 7 kDa
Observed band size: 7 kDaLanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-ATOX1 antibody [EPR10352] (ab154179) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 7 kDa -
ATOX1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 154179 at 1/20 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab154179 IP in HeLa whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab154179 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab154179 は 5 報の論文で使用されています。
- Alvarez-Rodriguez M et al. mRNA expression of oxidative-reductive proteins in boars with documented different fertility can identify relevant prognostic biomarkers. Res Vet Sci 141:195-202 (2021). PubMed: 34763256
- Wen MH et al. Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1. Stem Cell Res 41:101631 (2019). PubMed: 31704540
- Nie Y et al. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J 33:10705-10716 (2019). PubMed: 31262191
- Yee EMH et al. Dextran-Catechin inhibits angiogenesis by disrupting copper homeostasis in endothelial cells. Sci Rep 7:7638 (2017). PubMed: 28794411
- Blockhuys S & Wittung-Stafshede P Copper chaperone Atox1 plays role in breast cancer cell migration. Biochem Biophys Res Commun 483:301-304 (2017). WB ; Human . PubMed: 28027931