Anti-SCOP 抗体 (ab135957)
Key features and details
- Rabbit polyclonal to SCOP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SCOP antibody
SCOP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SCOP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human SCOP aa 1200-1250.
Sequence:D NREALYGVFD GDRNVEVPYL LQCTMSDILA EELQKTKNEE EYMVNTFIVM
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ポジティブ・コントロール
- Y79 cell extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135957の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 185 kDa.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 185 kDa. |
ターゲット情報
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機能
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts. -
組織特異性
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues. -
配列類似性
Contains 21 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
ドメイン
The PH domain is required for interaction with PRKCB and its dephosphorylation. -
細胞内局在
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 23239 Human
- Entrez Gene: 98432 Mouse
- Entrez Gene: 59265 Rat
- Omim: 609396 Human
- SwissProt: O60346 Human
- SwissProt: Q8CHE4 Mouse
- SwissProt: Q9WTR8 Rat
- Unigene: 465337 Human
see all -
別名
- hSCOP antibody
- KIAA0606 antibody
- PH domain and leucine rich repeat protein phosphatase antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab135957 は 2 報の論文で使用されています。
- Wang W et al. USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1/AKT Pathway. Biomed Res Int 2020:2509529 (2020). PubMed: 33029497
- Wang B et al. Silencing of TRIM11 suppresses the tumorigenicity of chordoma cells through improving the activity of PHLPP1/AKT. Cancer Cell Int 19:284 (2019). PubMed: 31719797