Anti-Annexin A1/ANXA1 抗体 [2F1] (ab135256)
Key features and details
- Mouse monoclonal [2F1] to Annexin A1/ANXA1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-Annexin A1/ANXA1 antibody [2F1]
Annexin A1/ANXA1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2F1] to Annexin A1/ANXA1 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human Annexin A1/ANXA1 aa 100-250.
Database link: P04083 -
ポジティブ・コントロール
- Human Annexin A1 recombinant protein; HeLa, A549, K562, NIH 3T3, C6 and COS7 cell lysates; Human cervical and ovarian cancer tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2F1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 39 kDa. |
IHC-P
1/200 - 1/1000. |
ターゲット情報
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機能
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
細胞内局在
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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参照データベース
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: P04083 Human
- SwissProt: P10107 Mouse
- SwissProt: P07150 Rat
- Unigene: 494173 Human
see all -
別名
- Annexin 1 antibody
- Annexin A1 antibody
- Annexin I (lipocortin I) antibody
see all
画像
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Annexin A1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab135256 observed at 38 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab135256 was shown to specifically react with Annexin A1 when Annexin A1 knockout samples were used. Wild-type and Annexin A1 knockout samples were subjected to SDS-PAGE. Ab135256 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
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Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution + Human Annexin A1 recombinant protein.
Predicted band size: 39 kDa
Expected MW of recombinant fragment is 33.7 kDa. -
All lanes : Anti-Annexin A1/ANXA1 antibody [2F1] (ab135256) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : COS7 cell lysate
Predicted band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling Annexin A1 with ab135256 at 1/200 dilution followed by DAB staining.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab135256 は論文での使用が確認できていません。