Anti-RhoGDI 抗体 [2G3] (ab135252)
Key features and details
- Mouse monoclonal [2G3] to RhoGDI
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-RhoGDI antibody [2G3]
RhoGDI 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2G3] to RhoGDI -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey
交差が予測される動物種: Cow -
免疫原
Purified recombinant full length Human RhoGDI (amino acids 1-204) expressed in E. coli.
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ポジティブ・コントロール
- Human RhoGDI recombinant protein; Jurkat, HeLa, NIH3T3, C6, K562 and COS7 cell lysates; HepG2 and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Protein stabilizer at 0.5%. -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2G3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 23 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 23 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
配列類似性
Belongs to the Rho GDI family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P19803 Cow
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
see all -
別名
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
画像
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Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution + Human RhoGDI recombinant protein.
Predicted band size: 23 kDa
Observed band size: 48.7 kDa why is the actual band size different from the predicted?
Note: the predicted band size of the full length protein is 23 kDa. The expected band size of the recombinant protein used here is 42.7 kDa. -
All lanes : Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C6 (Rat glial tumor cell line) cell lysate
Lane 5 : K562 cell lysate
Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Predicted band size: 23 kDa -
Immunofluorescence analysis of HepG2 cells using ab135252 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of HeLa cells using ab135252 at 1/200 dilution (green) and negative control (red).
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Immunofluorescence analysis of rat lung epithelial cells, staining RhoGDI with ab135252.
Cells were fixed with paraformaldehyde, permeabilized with 0.2% saponin and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 10% serum, 0.2% saponin in PBS) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab135252 は 1 報の論文で使用されています。
- Moretti AIS et al. Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families. Sci Rep 7:17262 (2017). WB ; Mouse . PubMed: 29222525