Anti-GFAP 抗体 (ab134436)
Key features and details
- Chicken polyclonal to GFAP
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-GFAP antibody
GFAP 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to GFAP -
由来種
Chicken -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
This product was produced with the following immunogens:
Recombinant full length protein corresponding to Cow GFAP. Recombinant full length protein followed by boosts of native GFAP protein purified from bovine spinal cords
Full length native protein (purified) corresponding to Cow GFAP. Recombinant full length protein corresponding to GFAP. Recombinant full length protein followed by boosts of native GFAP protein purified from bovine spinal cords -
特記事項
Do not freeze this antibody unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.5% BSA -
Concentration information loading...
-
精製度
IgY fraction -
特記事項(精製)
After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
|
特記事項 |
---|
ICC/IF
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
ターゲット情報
-
機能
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. -
組織特異性
Expressed in cells lacking fibronectin. -
関連疾患
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
Phosphorylated by PKN1. -
細胞内局在
Cytoplasm. Associated with intermediate filaments. - Information by UniProt
-
参照データベース
- Entrez Gene: 24387 Rat
- SwissProt: P47819 Rat
- Unigene: 91512 Rat
-
別名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
see all
画像
-
Immunofluorescent analysis of dissociated culture of rat cerebral cortical neurons and astrocytes labelling GFAP with ab134436 at 1/2000 dilution (red staining). The nuclei of some neuronal cells (blue staining) are visible.
-
ICC image of ab134436 stained primary neuron-gila rat cells. The cells were fixed with 3.7% formaldehyde in PBS (5 min), permeabilized with cold methanol and then blocked with 2% goat serum for 1h. The cells were then incubated with ab134436 (1/5000) overnight at +4°C followed by a further incubation at room termpature with Goat Anti-chicken IgG H&L (AlexaFluor® 488) at 1/2000 dilution (shown in red) for 1hr. Nuclear DNA was labelled in blue with DAPI.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (17)
ab134436 は 17 報の論文で使用されています。
- Lan G et al. Astrocytic VEGFA: An essential mediator in blood-brain-barrier disruption in Parkinson's disease. Glia 70:337-353 (2022). PubMed: 34713920
- Li H et al. Unbalanced regulation of Sec22b and Ykt6 blocks autophagosome axonal retrograde flux in neuronal ischemia-reperfusion injury. J Neurosci 42:5641-54 (2022). PubMed: 35654605
- Javonillo DI et al. Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease. Front Neurosci 15:785276 (2021). PubMed: 35140584
- Baglietto-Vargas D et al. Generation of a humanized Aß expressing mouse demonstrating aspects of Alzheimer's disease-like pathology. Nat Commun 12:2421 (2021). PubMed: 33893290
- Blackburn J et al. Astrocyte regional heterogeneity revealed through machine learning-based glial neuroanatomical assays. J Comp Neurol 529:2464-2483 (2021). PubMed: 33410136