Anti-MMP1 抗体 [EP1249Y] (ab134184)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1249Y] to MMP1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MMP1 antibody [EP1249Y]
MMP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1249Y] to MMP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.
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ポジティブ・コントロール
- WB: A431 cell lysate. IP: MDA-MB-231.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1249Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 54 kDa.
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IP |
1/20.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 54 kDa. |
IP
1/20. |
ターゲット情報
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機能
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4312 Human
- Omim: 120353 Human
- SwissProt: P03956 Human
- Unigene: 83169 Human
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別名
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
画像
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All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
Lane 2 : Lysate from HeLa cells untreated with TPA
Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
Lane 4 : Human colon cancer tissue lysate
Lane 5 : Human breast cancer tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST
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Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa -
MMP1 was immunoprecipitated from 0.35 mg MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with 134184 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2: ab134184 IP in MDA-MB-231 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab134184 in MDA-MB-231 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab134184 は 15 報の論文で使用されています。
- Dai Y et al. Dehydroevodiamine suppresses inflammatory responses in adjuvant-induced arthritis rats and human fibroblast-like synoviocytes. Bioengineered 13:268-279 (2022). PubMed: 34719315
- Zhu Y et al. Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT. Breast Cancer Res Treat 193:65-81 (2022). PubMed: 35254603
- Chen Q et al. Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an In Vitro Model of Actinic Dermatitis by Ultraviolet. Dis Markers 2022:5085183 (2022). PubMed: 36118675
- Mlakar L et al. Ameliorating Fibrosis in Murine and Human Tissues with END55, an Endostatin-Derived Fusion Protein Made in Plants. Biomedicines 10:N/A (2022). PubMed: 36359382
- Zhang J et al. lncRNA prostate cancer-associated transcript 18 upregulates activating transcription factor 7 to prevent metastasis of triple-negative breast cancer via sponging miR-103a-3p. Bioengineered 12:12070-12086 (2021). PubMed: 34787047