Anti-SLAM / CD150 抗体 [EPR5470] (ab134044)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5470] to SLAM / CD150
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SLAM / CD150 antibody [EPR5470]
SLAM / CD150 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5470] to SLAM / CD150 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SLAM/ CD150 aa 250-350. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Jurkat, HuT 78, MOLT4, Daudi and human thymus lysates. Flow Cyt (intra): Raji cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5470 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134044の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
組織特異性
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
翻訳後修飾
Phosphorylated by FYN. -
細胞内局在
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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別名
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
画像
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All lanes : Anti-SLAM / CD150 antibody [EPR5470] (ab134044) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : HuT 78 lysate
Lane 3 : MOLT4 lysate
Lane 4 : Daudi lysate
Lane 5 : Human thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa -
Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling SLAM / CD150 with ab134044 at 1/70 dilution, (11.13µg/ml) (red). Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488, Ab150077) was used as the secondary antibody at 1/2000. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabeled cells (Blue).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab134044 は 1 報の論文で使用されています。
- Chen Y et al. Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection. Theranostics 12:910-928 (2022). PubMed: 34976220