Human MMP 抗体 Array - Membrane (10 Targets) (ab134004)
製品の概要
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製品名
Human MMP Antibody Array - Membrane (10 Targets)
MMP Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Human -
製品の概要
ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types.
Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4
Antibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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特記事項
If you are interested in this antibody array, array ab169816 may also be of interest.
A table of human antibody membrane arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 2 x 25ml Cytokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134004の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
画像
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Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004.
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Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
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143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004.
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Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis.
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Membranes were probed with media and cell lysate from control and treated MCF-7 cells. These membranes were exposed to film for 1 minute.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab134004 は 14 報の論文で使用されています。
- Hsueh YH et al. Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model. J Orthop Translat 38:141-155 (2023). PubMed: 36381245
- Park J et al. Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritis. Exp Ther Med 22:808 (2021). PubMed: 34093764
- Ampey AC et al. Differential control of uterine artery endothelial monolayer integrity by TNF and VEGF is achieved through multiple mechanisms operating inside and outside the cell - Relevance to preeclampsia. Mol Cell Endocrinol 534:111368 (2021). PubMed: 34153378
- Triebel J et al. Matrix Metalloproteases and Cathepsin D in Human Serum do not Cleave Prolactin to Generate Vasoinhibin. Clin Lab 66:N/A (2020). PubMed: 32390374
- Dias F et al. Plasma Extracellular Vesicle-Derived TIMP-1 mRNA as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma: A Pilot Study. Int J Mol Sci 21:N/A (2020). PubMed: 32610589