Anti-Cathepsin L/V/K/H 抗体 [EPR8011] (ab133641)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cathepsin L/V/K/H antibody [EPR8011]
Cathepsin L/V/K/H 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISAmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Cathepsin L/V/K/H aa 100-200. The exact sequence is proprietary.
Database link: P07711 -
ポジティブ・コントロール
- WB: A549, HCT-116, NIH/3T3, PC-12 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 4.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8011 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133641の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
1/100.
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WB | (3) |
1/10000. Predicted molecular weight: 38 kDa.
For unpurified use at 1/1000 - 1/10000. |
IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. |
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
1/100. |
WB
1/10000. Predicted molecular weight: 38 kDa. For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
画像
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Antigen: CATL_HUMAN, CATV_HUMAN, CATS_HUMAN, CATK_HUMAN, CATH_HUMAN
Antigen concentration: 1000ng/ml
Primary antibody concentration range: 0~1000ng/ml
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Secondary antibody concentration: 1/2500
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ab133641 staining Cathepsin Lin the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 3 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin L recombinant protein fraction
Secondary
Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lane 2 : Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 38 kDaBlocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
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Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
Lane 6 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin V recombinant protein fraction
Secondary
Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDaBlocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
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Direct ELISA antigen dose-response curve using purified ab133641.Antigen concentration of 1000ng/mL.Alkaline Phosphatase conjugated AffiniPure goat anti-rabbit IgG(H+L)(1/2500) was used as the secondary antibody.
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Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/10000 dilution (purified)
Lane 1 : HCT-116 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified)
Lane 1 : A549 cell lysate
Lane 2 : HCT-116 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa -
Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified) + HepG2 cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with purified ab133641 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified ab133641 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Cathepsin L with purified ab133641 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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Unpurified ab133641 staining Cathepsin L in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 30 minutes. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab133641 は 15 報の論文で使用されています。
- Brun S et al. GNS561, a clinical-stage PPT1 inhibitor, is efficient against hepatocellular carcinoma via modulation of lysosomal functions. Autophagy 18:678-694 (2022). PubMed: 34740311
- Hirunsai M & Srikuea R Autophagy-lysosomal signaling responses to heat stress in tenotomy-induced rat skeletal muscle atrophy. Life Sci 275:119352 (2021). PubMed: 33771521
- Kim HJ et al. Crosstalk between HSPA5 arginylation and sequential ubiquitination leads to AKT degradation through autophagy flux. Autophagy 17:961-979 (2021). PubMed: 32164484
- Zhong B et al. Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Mol Ther 28:1078-1091 (2020). PubMed: 32053770
- Kwon I Protective effects of endurance exercise on skeletal muscle remodeling against doxorubicin-induced myotoxicity in mice. Phys Act Nutr 24:11-21 (2020). PubMed: 32698257