Optiblot SDS-PAGE Sample Preparation Kit (ab133414)
製品の概要
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製品名
Optiblot SDS-PAGE Sample Preparation Kit -
製品の概要
The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.
The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Optiblot SDS-PAGE Sample Preparation protocol is easily scaled up, allowing multiple samples to be prepared in parallel.
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アプリケーション
適用あり: Purificationmore details
製品の特性
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内容 10 tests 25 tests Extraction loading buffer 1 x 1ml 1 x 1ml Protein filters 10 units 25 units Resin 1 x 500ml 2 x 500ml -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133414の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Purification |
Use at an assay dependent concentration.
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特記事項 |
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Purification
Use at an assay dependent concentration. |
画像
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Resin concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Optiblot SDS-PAGE Sample Preparation Kit protocol (lane 4). The blots were detected using the Optiblot Fluorescent Western Blot Kit (ab133410). No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after resin concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab133414 は論文での使用が確認できていません。