Anti-mouse IgG for IP (HRP) (ab131368)
Key features and details
- Anti-mouse IgG for IP (HRP)
- Conjugation: HRP
- Host species: Rat
- Isotype: IgG
- Suitable for: WB
製品の概要
-
製品名
Anti-mouse IgG for IP (HRP)
IgG 二次抗体 製品一覧 -
由来種
Rat -
ターゲット生物種
Mouse -
アプリケーション
適用あり: WBmore details -
標識
HRP
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
eB144 -
アイソタイプ
IgG -
特記事項
Number of blots:
At least 20 blots, based on a 1 µl/ml dilution and 5 ml diluted antibody per blot.
Important protocol notes:
1. The anti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips -
研究分野
関連製品
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131368の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/1000 - 1/10000.
The optimal dilution will depend on the sensitivity of the HRP substrate. |
特記事項 |
---|
WB
1/1000 - 1/10000. The optimal dilution will depend on the sensitivity of the HRP substrate. |
画像
-
IP sample preparation: Caspase 7 was immunoprecipitated from 0.5 ml of 1x107 Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5 µg mouse anti-human Caspase 7.
WB conditions: Precipitate from 1x106 cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.
Detection:
Lane 1: Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368)
Lane 2: Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibody
Lane 3: Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (145)
ab131368 は 145 報の論文で使用されています。
- Forny P et al. Integrated multi-omics reveals anaplerotic rewiring in methylmalonyl-CoA mutase deficiency. Nat Metab 5:80-95 (2023). PubMed: 36717752
- Temizci B et al. Spastin Promotes the Migration and Invasion Capability of T98G Glioblastoma Cells by Interacting with Pin1 through Its Microtubule-Binding Domain. Cells 12:N/A (2023). PubMed: 36766769
- Pammi Guru KT et al. Isolation of Exosomes from Human Serum Using Gold-Nanoparticle-Coated Silicon Surface. Nanomaterials (Basel) 13:N/A (2023). PubMed: 36770347
- Spens AE et al. Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction. Elife 12:N/A (2023). PubMed: 37092726
- Bencurova M et al. Age-Dependent Changes in Calcium Regulation after Myocardial Ischemia-Reperfusion Injury. Biomedicines 11:N/A (2023). PubMed: 37189811