Anti-RPS6 抗体 (ab127980)
Key features and details
- Rabbit polyclonal to RPS6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RPS6 antibody
RPS6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPS6 -
由来種
Rabbit -
特異性
ab127980 detects endogenous levels of total S6 Ribosomal Protein protein. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide, corresponding to amino acids at the C-terminus of Human RPS6.
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ポジティブ・コントロール
- Human Pancreas tissue; HeLa cell extracts, treated with TNF-a 20ng/ml; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ or Ca2+ . -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab127980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
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IHC-P |
Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa. |
IHC-P
Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
配列類似性
Belongs to the ribosomal protein S6e family. -
翻訳後修飾
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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参照データベース
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 29304 Rat
- Omim: 180460 Human
- SwissProt: P62753 Human
- SwissProt: P62754 Mouse
- SwissProt: P62755 Rat
see all -
別名
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
画像
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All lanes : Anti-RPS6 antibody (ab127980) at 1/500 dilution
Lane 1 : HeLa cell extract, treated with TNF-a 20ng/ml for 2 minutes
Lane 2 : HeLa cell extract, treated with the synthesized peptide.
Developed using the ECL technique.
Predicted band size: 29 kDa -
ab127980 at 1/100 dilution, staining RPS6 in HeLa cells by Immunofluorescence. The image on the right is treated with synthesized peptide.
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ab127980 at 2.5µg/ml, staining RPS6 in Formalin-Fixed, Paraffin-Embedded Human pancreas tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab127980 は論文での使用が確認できていません。