Anti-UBXN2B 抗体 [OTI3H8] (ab124032)
Key features and details
- Mouse monoclonal [OTI3H8] to UBXN2B
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-UBXN2B antibody [OTI3H8] -
製品の詳細
Mouse monoclonal [OTI3H8] to UBXN2B -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human UBXN2B aa 2-331. Produced in HEK293T cells (NP_001071087).
Sequence:AEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKA TVFKSPRTPPQRFYSSEHEYSGLNIVRPSTGKIVNELFKEAREHGAVPLN EATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQDVQILLKLWSN GFSLDDGELRPYNEPTNAQFLESVKRGEIPLELQRLVHGGQVNLDMEDHQ DQEYIKPRLRFKAFSGEGQKLGSLTPEIVSTPSSPEEEDKSILNAVVLID DSVPTTKIQIRLADGSRLIQRFNSTHRILDVRNFIVQSRPEFAALDFILV TSFPNKELTDESLTLLEADILNTVLLQQLK
Database link: Q14CS0 -
ポジティブ・コントロール
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY UBXN2B cDNA; HepG2, HeLa, HT29, A549, COS-7, Jurkat, MDCK and MCF7 cell extracts Flow Cyt (Intra): HEK293T cells transfected with a UBXN2B overexpress plasmid
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI3H8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 37 kDa.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/2000. Predicted molecular weight: 37 kDa. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Adapter protein required for Golgi and endoplasmic reticulum biogenesis. Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis. The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L. VCPIP1 is also required, but not its deubiquitinating activity. -
配列類似性
Belongs to the NSFL1C family.
Contains 1 SEP domain.
Contains 1 UBX domain. -
細胞内局在
Nucleus. Cytoplasm > cytosol. Endoplasmic reticulum. Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 137886 Human
- Omim: 610686 Human
- SwissProt: Q14CS0 Human
- Unigene: 155572 Human
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別名
- NSFL1 cofactor p37 antibody
- p37 antibody
- p97 cofactor p37 antibody
see all
画像
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All lanes : Anti-UBXN2B antibody [OTI3H8] (ab124032) at 1/1000 dilution
Lane 1 : Human testis tissue extract
Lane 2 : Human omentum tissue extract
Lane 3 : Human uterus tissue extract
Lane 4 : Human breast tissue extract
Lane 5 : Human brain tissue extract
Lane 6 : Human liver tissue extract
Lane 7 : Human ovary tissue extract
Lane 8 : Human thyroid gland tissue extract
Lane 9 : Human colon tissue extract
Lane 10 : Human spleen tissue extract
Predicted band size: 37 kDa -
All lanes : Anti-UBXN2B antibody [OTI3H8] (ab124032) at 1/1000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY UBXN2B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 37 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205243) -
ab124032, at a 1/100 dilution, staining UBXN2B in HEK293T cells transfected with a UBXN2B overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab124032 は論文での使用が確認できていません。