trans-Retinoic acid, Stem cell differentiator (ab120728)
Key features and details
- Vitamin A metabolite. Stem cell differentiator.
- CAS Number: 302-79-4
- Purity: > 99%
- Soluble in DMSO to 25 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
trans-Retinoic acid, Stem cell differentiator -
製品の詳細
Vitamin A metabolite. Stem cell differentiator. -
別名
- ATRA
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精製度
> 99% -
CAS 番号
302-79-4 -
構造式
製品の特性
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体系名
3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2E,4E,6E,8E-nonatetraenoic acid -
分子量
300.44 -
分子式
C20H28O2 -
保存方法
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 25 mM and in ethanol to 10 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120728の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120728, trans-Retinoic acid, Stem cell differentiator
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ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid (ab120728), by ICC/IF. Decrease of Oct4 expression correlates with increased concentration of trans-retinoic acid, as described in literature.
The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19857 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (4)
ab120728 は 4 報の論文で使用されています。
- Li Z et al. Transcriptomics of Hirschsprung disease patient-derived enteric neural crest cells reveals a role for oxidative phosphorylation. Nat Commun 14:2157 (2023). PubMed: 37061531
- Dolce LG et al. Myosin Va interacts with the exosomal protein spermine synthase. Biosci Rep 39:N/A (2019). PubMed: 30733278
- Maina MB et al. The involvement of tau in nucleolar transcription and the stress response. Acta Neuropathol Commun 6:70 (2018). PubMed: 30064522
- Zhou TB et al. Effect of all-trans retinoic acid treatment on prohibitin and renin-angiotensin-aldosterone system expression in hypoxia-induced renal tubular epithelial cell injury. J Renin Angiotensin Aldosterone Syst 15:243-9 (2014). PubMed: 25031298