RTIL™ 13, dynamin I and II inhibitor (ab120465)
Key features and details
- Potent dynamin I and II inhibitor
- CAS Number: 1009376-10-6
- Soluble in DMSO to 20 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
RTIL™ 13, dynamin I and II inhibitor -
製品の詳細
Potent dynamin I and II inhibitor -
生理活性の詳細
Potent dynamin I and II inhibitor. Inhibits dynamin I GTPase (IC50 = 2.3 μM) and targets pleckstrin homology (PH) (lipid binding) domain. Inhibits receptor-mediated and synaptic vesicle endocytosis (IC50 values are 9.3 and 7.1 μM, respectively).
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特記事項
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. RTIL™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd. -
CAS 番号
1009376-10-6 -
構造式
製品の特性
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体系名
4-(N,N-Dimethyl-N-octadecyl-N-ethyl)-4-aza-10-oxatricyclo-[5.2.1]decane-3,5-dione bromide -
分子量
571.68 -
分子式
C30H55BrN2O3 -
保存方法
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 20 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120465, RTIL™ 13, dynamin I and II inhibitor
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ab13847 staining active caspase 3 in HeLa cells treated with RTIL-13™ (ab120465), by ICC/IF. Increase in active caspase 3 expression correlates with increased concentration of RTIL-13™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120465 (RTIL-13™) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab120465 は論文での使用が確認できていません。