PP2, Src and RIP2 kinase inhibitor (ab120308)
Key features and details
- Src and RIP2 kinase inhibitor
- CAS Number: 172889-27-9
- Soluble in DMSO to 50 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
PP2, Src and RIP2 kinase inhibitor -
製品の詳細
Src and RIP2 kinase inhibitor -
CAS 番号
172889-27-9 -
構造式
製品の特性
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体系名
1-tert-Butyl-3-(4-chlorophenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine -
分子量
301.78 -
分子式
C15H16ClN5 -
PubChem 登録番号
4878 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 50 mM and in ethanol to 10 mM -
使用に関する注意
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
Clc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120308の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120308, PP2, Src and RIP2 kinase inhibitor
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ab11512 staining E-Cadherin in SW480 cells treated with PP2 (ab120308), by ICC/IF. Increase in E-cadherin expression correlates with increased concentration of PP2, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120308 (PP2) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab96971) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (14)
ab120308 は 14 報の論文で使用されています。
- Chen Z et al. Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway. Mediators Inflamm 2021:6639252 (2021). PubMed: 33927570
- Yoo JY et al. LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1. Cell Rep 33:108245 (2020). PubMed: 33086058
- Nasyrov E et al. The neuronal oxygen-sensing pathway controls postnatal vascularization of the murine brain. FASEB J 33:12812-12824 (2019). PubMed: 31469589
- Fukushima T et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep 29:4144-4158.e7 (2019). PubMed: 31851939
- Tischer DK & Weiner OD Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. Elife 8:N/A (2019). PubMed: 30947808