Fluoxetine hydrochloride, 5-HT reuptake inhibitor (ab120077)
Key features and details
- 5-HT reuptake inhibitor
- CAS Number: 56296-78-7
- Soluble in water to 10 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Fluoxetine hydrochloride, 5-HT reuptake inhibitor -
製品の詳細
5-HT reuptake inhibitor -
生理活性の詳細
Potent, selective 5-HT reuptake inhibitor. Antidepressant in vivo. Binds to rat and human 5-HT transporters with Ki values of 2 and 0.9-20 nM, respectively. Shows around 150-900-fold selectivity over 5-HT1A, 5-HT2A, H1, α1, α2-adrenergic and muscarinic receptors.
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CAS 番号
56296-78-7 -
構造式
製品の特性
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体系名
N-Methyl-3-[(4-trifluoromethyl)phenoxy]-3-phenylpropylamine hydrochloride -
分子量
345.79 -
分子式
C17H18F3NO.HCl -
PubChem 登録番号
62857 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 10 mM and in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
CNCCC(C1=CC=CC=C1)OC2=CC=C(C=C2)C(F)(F)F.Cl -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120077, Fluoxetine hydrochloride, 5-HT reuptake inhibitor
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ab66047 staining serotonin in SKNSH cells treated with fluoxetine hydrochloride (ab120077), by ICC/IF. Increase of serotonin expression correlates with increased concentration of fluoxetine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120077 (fluoxetine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66047 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 donkey anti-goat polyclonal antibody (ab96931) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (5)
ab120077 は 5 報の論文で使用されています。
- Nollet M Models of Depression: Unpredictable Chronic Mild Stress in Mice. Curr Protoc 1:e208 (2021). PubMed: 34406704
- Aller MI et al. Increased Dosage of High-Affinity Kainate Receptor Gene grik4 Alters Synaptic Transmission and Reproduces Autism Spectrum Disorders Features. J Neurosci 35:13619-28 (2015). PubMed: 26446216
- Casal-Dominguez JJ et al. In vivo and in vitro characterization of naltrindole-derived ligands at the ?-opioid receptor. J Psychopharmacol 27:192-202 (2013). PubMed: 23118019
- Greenhill SD & Jones RS Diverse antiepileptic drugs increase the ratio of background synaptic inhibition to excitation and decrease neuronal excitability in neurones of the rat entorhinal cortex in vitro. Neuroscience 167:456-74 (2010). PubMed: 20167261
- Toulme E et al. P2X4 receptors in activated C8-B4 cells of cerebellar microglial origin. J Gen Physiol 135:333-53 (2010). PubMed: 20231374