NBQX, AMPA / kainate antagonist (ab120045)
Key features and details
- AMPA / kainate antagonist
- CAS Number: 118876-58-7
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
NBQX, AMPA / kainate antagonist -
製品の詳細
AMPA / kainate antagonist -
生理活性の詳細
Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).
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CAS 番号
118876-58-7 -
構造式
製品の特性
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体系名
2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide -
分子量
336.28 -
分子式
C12H8N4O6S -
PubChem 登録番号
3272524 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in DMSO to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
[O-][N+](=O)c2cc3NC(=O)C(=O)Nc3c1cccc(c12)S(N)(=O)=O -
由来
Synthetic
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研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
画像
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2D chemical structure image of ab120045, NBQX, AMPA / kainate antagonist
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A - Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom).
B - Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom)
Both cells were held at −68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 µm D-AP5 and 10 µm NBQX (ab120045, traces in red). Each trace represents an average of 10–20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar: (A) 50 µm, (B) 20 µm
Credit: Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955
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ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (134)
ab120045 は 134 報の論文で使用されています。
- Lin C et al. Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators. Cell Rep Methods 3:100544 (2023). PubMed: 37671014
- Anstötz M et al. Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors. Cell Rep 39:110822 (2022). PubMed: 35584670
- Kolling LJ et al. Modulating the Excitability of Olfactory Output Neurons Affects Whole-Body Metabolism. J Neurosci 42:5966-5990 (2022). PubMed: 35710623
- Vaden RJ et al. Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells. Elife 9:N/A (2020). PubMed: 32602839
- Goz RU et al. BRAFV600E expression in neural progenitors results in a hyperexcitable phenotype in neocortical pyramidal neurons. J Neurophysiol 123:2449-2464 (2020). PubMed: 32401131