D-AP5, NMDA glutamate site antagonist (ab120003)
Key features and details
- NMDA glutamate site antagonist
- CAS Number: 79055-68-8
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
D-AP5, NMDA glutamate site antagonist -
製品の詳細
NMDA glutamate site antagonist -
別名
- APV
- D-APV
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生理活性の詳細
Competitive NMDA receptor glutamate site antagonist. More active form of DL-AP5.
Also available in simple stock solutions (ab144482) - add 1 ml of water to get an exact, ready-to-use concentration.
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CAS 番号
79055-68-8 -
構造式
製品の特性
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体系名
D-(-)-2-Amino-5-phosphonopentanoic acid -
分子量
197.13 -
分子式
C5H12NO5P -
PubChem 登録番号
135342 -
保存方法
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
N[C@H](CCCP(=O)(O)O)C(=O)O -
由来
Synthetic
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研究分野
画像
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2D chemical structure image of ab120003, D-AP5, NMDA glutamate site antagonist
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(A) Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom). (B) Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom). Both cells were held at -68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 µm D-AP5 and 10 µm NBQX (traces in red). Each trace represents an average of 10-20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar: (A) 50 µm, (B) 20 µm.
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Averaged Ca2+ transients (500 Hz line scans) evoked by 40 ms voltage step in a dendrite (left) and spine (right) in control (black), D-AP5 (red, 10 µM), after a 10 min washout of D-AP5 (green), and in 5 µM NMDA (blue). Mibefradil (20 µM), nimodipine (20 µM) and TTX (0.5 µM) were present throughout.
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Representative voltage-clamp recording (Vh= −60 mV, ECl−=+8 mV) of a Purkinje cells response to simulated ischemia and sequential block of glutamate receptors and GABAA receptors.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (267)
ab120003 は 267 報の論文で使用されています。
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- Lenz M et al. Denervated mouse CA1 pyramidal neurons express homeostatic synaptic plasticity following entorhinal cortex lesion. Front Mol Neurosci 16:1148219 (2023). PubMed: 37122623
- Katada Y et al. Starburst amacrine cells amplify optogenetic visual restoration through gap junctions. Mol Ther Methods Clin Dev 30:1-13 (2023). PubMed: 37324975
- Lin C et al. Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators. Cell Rep Methods 3:100544 (2023). PubMed: 37671014
- Boinon L et al. Conditional knockout of CRMP2 in neurons, but not astrocytes, disrupts spinal nociceptive neurotransmission to control the initiation and maintenance of chronic neuropathic pain. Pain 163:e368-e381 (2022). PubMed: 35029600