Mouse IL-1ra ELISA Kit (ab113348)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-1ra ELISA Kit
IL-1RA キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 2 pg/ml -
検出範囲
2.74 pg/ml - 2000 pg/ml -
添加回収試験
90 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 102 94% - 103% Serum 75.89 67% - 84% Plasma 93.69 71% - 111% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human -
製品の概要
Abcam’s IL-1ra Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-1ra in serum, plasma (collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended for this assay) and cell culture supernates.
This assay employs an antibody specific for mouse IL-1ra coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1ra present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1ra antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1ra bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated anti-Mouse IL-1ra 2 vials IL-1ra Microplate (12 x 8 wells) 1 unit Recombinant Mouse IL-1ra Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. -
組織特異性
The intracellular form of IL1RN is predominantly expressed in epithelial cells. -
関連疾患
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency -
細胞内局在
Cytoplasm and Secreted. - Information by UniProt
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別名
- DIRA
- F630041P17Rik
- ICIL 1RA
see all -
参照データベース
- Entrez Gene: 3557 Human
- Entrez Gene: 16181 Mouse
- Entrez Gene: 60582 Rat
- Omim: 147679 Human
- SwissProt: P18510 Human
- SwissProt: P25085 Mouse
- SwissProt: P25086 Rat
- Unigene: 81134 Human
see all
画像
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Standard curve of msIL-1Ra in different diluents with background signal subtracted (duplicates; +/- SD).
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IL-1Ra measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
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msIL-1Ra measured in biological fluids, background signal subtracted (duplicates +/- SD).
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IL-1Ra detected in supernatants from RAW 246.7 control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), or 24 hours with PMA and 1 ug x mL-1 of LPS (Sigma) (P+L) for the last 6 hours. Results shown after background signal was subtracted (duplicates +/- SD).
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Representative Standard Curve using ab113348
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Representative Standard Curve using ab113348
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab113348 は 1 報の論文で使用されています。
- Sakkou M et al. Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis. JCI Insight 3:N/A (2018). PubMed: 29618659