Maleimide Quantification Assay Kit (Colorimetric) (ab112140)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Suspension cells
- Sensitivity: 10 pg/well
製品の概要
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製品名
Maleimide Quantification Assay Kit (Colorimetric)
Maleimide キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture extracts, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
10 pg/well -
製品の概要
ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit ab112141 that has higher sensitivity.
Visit our FAQs page for tips and troubleshooting. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 100 tests 4,4’-DTDP Component B 1 vial Assay Buffer 1 x 50ml DMSO 1 x 1ml MEA Component A 1 vial -
研究分野
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab112140 は論文での使用が確認できていません。