Anti-Epac1 抗体 [EPR1672] (ab109415)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1672] to Epac1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Epac1 antibody [EPR1672]
Epac1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1672] to Epac1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, HepG2, Human heart and HUVEC lysates; Human kidney tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1672 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 104 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ターゲット情報
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関連性
The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography. -
細胞内局在
Endomembrane system -
参照データベース
- Entrez Gene: 10411 Human
- Entrez Gene: 223864 Mouse
- Entrez Gene: 59326 Rat
- Omim: 606057 Human
- SwissProt: O95398 Human
- SwissProt: Q8VCC8 Mouse
- SwissProt: Q9Z1C8 Rat
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別名
- bcm910 antibody
- CAMP GEFI antibody
- cAMP regulated guanine nucleotide exchange factor I antibody
see all
画像
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All lanes : Anti-Epac1 antibody [EPR1672] (ab109415) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human heart lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 104 kDa -
Immunohistochemical analysis of Epac1 in paraffin-embedded Human kidney tissue using ab109415 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab109415 は 5 報の論文で使用されています。
- Xu XH et al. The role of G-protein-coupled receptor kinase 2 in diabetic mechanical hyperalgesia in rats. Eur J Pain 25:2039-2049 (2021). PubMed: 34101933
- Zhang B et al. Arecoline Enhances Phosphodiesterase 4A Activity to Promote Transforming Growth Factor-β-Induced Buccal Mucosal Fibroblast Activation via cAMP-Epac1 Signaling Pathway. Front Pharmacol 12:722040 (2021). PubMed: 34819854
- García-Ponce A et al. Epac1 Is Crucial for Maintenance of Endothelial Barrier Function through A Mechanism Partly Independent of Rac1. Cells 9:N/A (2020). PubMed: 32992982
- Liu L et al. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis 23:1-7 (2017). PubMed: 28210097
- Wang SY et al. Sex Steroids Influence Brain-Derived Neurotropic Factor Secretion From Human Airway Smooth Muscle Cells. J Cell Physiol 231:1586-92 (2016). PubMed: 26566264