Anti-TYRO3 抗体 [EPR4308] (ab109231)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4308] to TYRO3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TYRO3 antibody [EPR4308]
TYRO3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4308] to TYRO3 -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Fetal brain, fetal hippocampus, MCF7, Rat hippocampus, Rat brain, Human fetal brain, and Mouse hippocampus lysates IP: SH-SY5Y whole cell lysate IHC-P: Human brain tissue, Human colon carcinoma and Human ovarian carcinoma
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4308 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109231の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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IP |
1/200.
For unpurified use at 1/10 - 1/100. |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
IP
1/200. For unpurified use at 1/10 - 1/100. |
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
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機能
May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor. -
組織特異性
Abundant in the brain and lower levels in other tissues. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7301 Human
- Entrez Gene: 22174 Mouse
- Entrez Gene: 25232 Rat
- Omim: 600341 Human
- SwissProt: Q06418 Human
- SwissProt: P55144 Mouse
- SwissProt: P55146 Rat
- Unigene: 381282 Human
see all -
別名
- Brt antibody
- BYK antibody
- DTK antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified)
Lane 1 : Rat hippocampus lysates at 20 µg
Lane 2 : Rat brain lysates at 20 µg
Lane 3 : Human fetal brain lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified) + Mouse hippocampus lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
ab109231 (purified) at 1:200 dilution (2µg) immunoprecipitating TYRO3 in SH-SY5Y whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109231 & SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109231 in SH-SY5Y whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:2000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of TYRO3 in paraffin embedded Human brain tissue, using ab109231 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution
Lane 1 : fetal brain lysates
Lane 2 : fetal hippocampus lysates
Lane 3 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 97 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab109231 は 11 報の論文で使用されています。
- Zhong F et al. TYRO3 agonist as therapy for glomerular disease. JCI Insight 8:N/A (2023). PubMed: 36454644
- Vullings J et al. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis. J Immunol Res 2020:9690832 (2020). PubMed: 32964059
- Liu H et al. Endothelial specific YY1 deletion restricts tumor angiogenesis and tumor growth. Sci Rep 10:20493 (2020). PubMed: 33235311
- Li XL et al. Rapamycin Alleviates the Symptoms of Multiple Sclerosis in Experimental Autoimmune Encephalomyelitis (EAE) Through Mediating the TAM-TLRs-SOCS Pathway. Front Neurol 11:590884 (2020). PubMed: 33329339
- Du J et al. circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis. Cancer Cell Int 20:430 (2020). PubMed: 32908453