Anti-Wnt2/IRP 抗体 [EPR3101(2)] (ab109222)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3101(2)] to Wnt2/IRP
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Wnt2/IRP antibody [EPR3101(2)]
Wnt2/IRP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3101(2)] to Wnt2/IRP -
由来種
Rabbit -
アプリケーション
適用あり: WB, Indirect ELISAmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab197186) -
ポジティブ・コントロール
- WB: Jurkat, MCF-7,T47-D, and SKBR-3 cell lysates. Rat and mouse brain tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Frozen Stock (-20C). Shelf life 12 months. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3101(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109222の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa).
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Indirect ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 40 kDa). |
Indirect ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
組織特異性
Expressed in brain in the thalamus, in fetal and adult lung and in placenta. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7472 Human
- Entrez Gene: 22413 Mouse
- Entrez Gene: 114487 Rat
- Omim: 147870 Human
- SwissProt: P09544 Human
- SwissProt: P21552 Mouse
- Unigene: 567356 Human
- Unigene: 33653 Mouse
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製品の状態
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. -
別名
- Int 1 related protein antibody
- Int-1-like protein 1 antibody
- Int-1-related protein antibody
see all
画像
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All lanes : Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : SKBR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Representative standard curve using ab109222
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Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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All lanes : Anti-Wnt2/IRP antibody [EPR3101(2)] (ab109222) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysates
Lane 2 : MCF-7 cell lysates
Lane 3 : T47-D cell lysates
Lane 4 : SKBR-3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab109222 は 14 報の論文で使用されています。
- Yu H et al. SNHG12 promotes carcinogenesis of human renal cell cancer via functioning as a competing endogenous RNA and sponging miR-30a-3p. J Cell Mol Med 25:4696-4708 (2021). PubMed: 33787057
- Xie B et al. MicroRNA-1246 by Targeting AXIN2 and GSK-3ß Overcomes Drug Resistance and Induces Apoptosis in Chemo-resistant Leukemia Cells. J Cancer 12:4196-4208 (2021). PubMed: 34093820
- Zou S et al. ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR-8/SVneo cells via modulating the epithelial-mesenchymal transition. J Biochem Mol Toxicol 34:e22454 (2020). PubMed: 31981282
- Guan G et al. Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin-induced in vitro ototoxicity in HEI-OC1 auditory cells using gene expression profiling. Mol Med Rep 22:5053-5068 (2020). PubMed: 33174043
- Chen Q et al. Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression. BMC Mol Cell Biol 21:80 (2020). PubMed: 33167868