Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108799)
Key features and details
- Sensitivity: 0.31 ng/ml
- Range: 0.391 ng/ml - 25 ng/ml
- Sample type: Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)
alpha 1 Antitrypsin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.3% -
サンプルの種類
Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.31 ng/ml -
検出範囲
0.391 ng/ml - 25 ng/ml -
添加回収試験
103 %
-
全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human alpha 1 Antitrypsin ELISA kit (SERPINA1) (ab108799) is a sandwich ELISA kit for the quantitative measurement of alpha 1 Antitrypsin in plasma, serum, milk, urine, saliva and CSF samples.
An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.
Get results in 90 minutes with Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human alpha 1 Antitrypsin Antibody 1 x 150µl alpha 1 Antitrypsin Microplate (12 x 8 well strips) 1 unit alpha 1 Antitrypsin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
-
機能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
組織特異性
Ubiquitous. Expressed in leukocytes and plasma. -
関連疾患
Alpha-1-antitrypsin deficiency -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻訳後修飾
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
細胞内局在
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
-
別名
- A1A
- A1AT
- A1AT_HUMAN
see all -
参照データベース
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
関連製品
-
SimpleStep ELISA kits
画像
-
Linearity: alpha 1 antitrypsin in urine at different diutions, corrected for the factor of dilution (duplicates +/- SD).
-
Linearity: alpha 1 antitrypsin in human milk at different diutions, corrected for the factor of dilution (duplicates +/- SD)
-
Linearity: alpha 1 antitrypsin in serum and plasma (Li-heparine) at different diutions, corrected for the factor of dilution (duplicates +/- SD)
-
Alpha 1 antitrypsin in human biological fluids (duplicates +/- SD).
The samples were tested in different ranges of dilutions (serum and Li-heparine plasma: 2e3-1e6; CSF: 5e3-5e4; milk: 1e2-1e3; saliva: 2e2-2e3).
-
Representative Standard Curve using ab108799
-
Standard curve with background signal subtracted (duplicates; +/- SD).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (20)
ab108799 は 20 報の論文で使用されています。
- Kang HK et al. Establishing a 3D In Vitro Hepatic Model Mimicking Physiologically Relevant to In Vivo State. Cells 10:N/A (2021). PubMed: 34065411
- Karadagi A et al. Systemic modified messenger RNA for replacement therapy in alpha 1-antitrypsin deficiency. Sci Rep 10:7052 (2020). PubMed: 32341402
- Subramani PA et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333
- Michielin F et al. The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs. Cell Rep 33:108453 (2020). PubMed: 33264615
- Jain S et al. Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization. Nitric Oxide 95:1-11 (2020). PubMed: 31778801